NAD(P)H- and superoxide-dependent nitric oxide degradation by rat liver mitochondria

Autor(es)

Oliveira, Halley Caixeta ^[1] ; Saviani, Elzira Elisabeth ^[1] ; Salgado, Ione ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Biol Vegetal, Inst Biol, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FEBS Letters; v. 583, n. 13, p. 2276-2280, JUL 7 2009.
• Citações Web of Science: 1

Resumo

Mitochondria consume nitric oxide (NO) mainly through reaction with superoxide anion (O(2)(-)). Here, we analyzed the O(2)(-) sources for NO degradation by isolated rat liver mitochondria. Electron leakage from complex III and reverse electron transport to complex I accounted for O(2)(-) -dependent NO degradation by mitochondria in the presence of a protonmotive force. Mitochondria incubated with NAD(P) H also presented intense O(2)(-) generation and NO degradation rates that were insensitive to respiratory inhibitors and abolished after proteinase treatment. These results suggest that an outer membrane-localized NAD(P) H oxidase activity, in addition to the electron leakage from the respiratory chain, promotes O(2)(-) -dependent NO degradation in rat liver mitochondria. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved. (AU)

• Processo FAPESP: 05/54246-4 - Sintese, sinalizacao e alvos mitocondriais do radical oxido nitrico em vegetais.
• Beneficiário: Ione Salgado
• Modalidade de apoio: Auxílio à Pesquisa - Regular