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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

NAD(P)H- and superoxide-dependent nitric oxide degradation by rat liver mitochondria

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Author(s):
Oliveira, Halley Caixeta [1] ; Saviani, Elzira Elisabeth [1] ; Salgado, Ione [1]
Total Authors: 3
Affiliation:
[1] Univ Estadual Campinas, Dept Biol Vegetal, Inst Biol, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: FEBS Letters; v. 583, n. 13, p. 2276-2280, JUL 7 2009.
Web of Science Citations: 1
Abstract

Mitochondria consume nitric oxide (NO) mainly through reaction with superoxide anion (O(2)(-)). Here, we analyzed the O(2)(-) sources for NO degradation by isolated rat liver mitochondria. Electron leakage from complex III and reverse electron transport to complex I accounted for O(2)(-) -dependent NO degradation by mitochondria in the presence of a protonmotive force. Mitochondria incubated with NAD(P) H also presented intense O(2)(-) generation and NO degradation rates that were insensitive to respiratory inhibitors and abolished after proteinase treatment. These results suggest that an outer membrane-localized NAD(P) H oxidase activity, in addition to the electron leakage from the respiratory chain, promotes O(2)(-) -dependent NO degradation in rat liver mitochondria. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved. (AU)