Mast cell concentration in the wound healing process of incisions made by different instruments

Autor(es)

Azevedo, Luciane H. ^{[1, 2]} ; de Sousa, Suzana C. O. M. ^[3] ; Correa, Luciana ^[4] ; de Paula Eduardo, Carlos ^{[5, 1]} ; Zaidan Dagli, Maria Lucia ^[6] ; Romanos, Georgios ^[7] ; Migliari, Dante A. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Dent, Special Lab Lasers Dent LELO, BR-05508900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Dent, Dept Oral Diag, BR-05508900 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Dent, Dept Oral Pathol, BR-05508900 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Sch Dent, Dept Pathol, BR-05508900 Sao Paulo - Brazil

^[5] Univ Sao Paulo, Sch Dent, Dept Restorat Dent, BR-05508900 Sao Paulo - Brazil

^[6] Univ Sao Paulo, Fac Vet Med, Dept Pathol, BR-05508900 Sao Paulo - Brazil

^[7] Univ Rochester, Med Ctr, Dept Periodontol & Gen Dent, Rochester, NY 14642 - USA

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 24, n. 4, p. 585-590, JUL 2009.
• Citações Web of Science: 7

Resumo

The aim of this study was to compare the concentration of mast cells (MCs) in the healing process of incisions. Thirty rats were submitted to six linear incisions each, performed in the dorsal skin by carbon dioxide (CO(2)) and diode lasers, electrocautery and conventional scalpel. The animals were euthanized at intervals of 0 h, 24 h, 48 h, 72 h, 7 days and 14 days after the incisions had been made. Histological sections were obtained and stained with toluidine blue for identification of MCs, which were manually counted by conventional microscopy in 20 microscopic fields in the border of the incision, near the granulation tissue, or in the area of new collagen formation, depending on intervals. The concentration of MCs was significantly higher in the wounds made by scalpel than in those made by other techniques at 48 h and 72 h. After 72 h the number of MCs was also significantly higher after electrocautery than after incisions made by 4 W CO(2) laser. On days 7 and 14, there was no significant difference in the MC count among the different types of incisions. In summary, the MC concentration varied after different surgical incisions at early phases of wound healing. At the end of the healing process, however, there were similar MC concentrations around the incisions, suggesting that, in standard incisions in the surgical techniques studied, the wound healing process ultimately occurred in a similar pattern. (AU)

• Processo FAPESP: 04/06794-0 - Processo reparacional em tecido cutâneo e oral de ratos submetidos a incisão cirúrgica com lasers de CO2 e diodo, bisturi elétrico e convencional: uma análise morfológica, histoquímica e imuno-histoquímica
• Beneficiário: Suzana Cantanhede Orsini Machado de Sousa
• Modalidade de apoio: Auxílio à Pesquisa - Regular