Peptidase activities in the semen from the ductus deferens and uterus of the neotropical rattlesnake Crotalus durissus terrificus

Autor(es)

Marinho, Camila Eduardo ^{[1, 2]} ; Almeida Santos, Selma Maria ^[3] ; Yamasaki, Simone Cristina ^[1] ; Silveira, Paulo Flavio ^[1]

Afiliação do(s) autor(es)

^[1] Inst Butantan, Pharmacol Lab, BR-05503900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biociencias, Dept Physiol, BR-05508900 Sao Paulo - Brazil

^[3] Inst Butantan, Lab Ecol & Evolut, BR-05503900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL; v. 179, n. 5, p. 635-642, JUL 2009.
• Citações Web of Science: 5

Resumo

To understand the role of peptidases in seminal physiology of Crotalus durissus terrificus, intra- and inter-seasonal activity levels of acid (APA), basic (APB), puromycin-sensitive (APN-PS) and puromycin-insensitive neutral (APN-PI), cystyl (CAP), dipeptidyl-IV (DPPIV), type-1 pyroglutamyl (PAP-I) and prolyl-imino (PIP) aminopeptidases as well as prolyl endopeptidase (POP) were evaluated in soluble (SF) and/or membrane-bound (MF) fractions of semen collected from the ductus deferens of the male reproductive tract and from the posterior portion of the uterus. Seminal APB, PIP and POP were detected in SF, while other peptidases were detected in SF and MF. Only the convoluted posterior uterus in winter and autumn had semen. Relative to other examined peptidases, in general, APN-PI, APN-PS and APB activities were predominant in the semen from the uterus and throughout the year in the semen from the ductus deferens, suggesting their great relevance in the seminal physiology of C. d. terrificus. The levels of peptidase activities in the ductus deferens semen varied seasonally and were different from those of semen in the uterus, suggesting that their modulatory actions on susceptible peptides are integrated to the male reproductive cycle events and spermatozoa viability of this snake. (AU)

• Processo FAPESP: 07/06829-6 - Efeitos do veneno de Apis mellifera sobre anticorpo anti-colágeno tipo II, interleucina 6 e peptidases do líquido e membrana sinoviais e de leucócitos circulantes em ratos com artrite induzida por colágeno tipo II
• Beneficiário: Simone Cristina Yamasaki
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 04/14971-9 - Peptidases do semen, da vagina, do utero e do vas deferens: possivel relacao com a sobrevida de espermatozoides da cascavel crotalus durissus terrificus (viperidae: crotalinae).
• Beneficiário: Paulo Flávio Silveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/03745-0 - A cascavel Crotalus durissus terrificus (Viperidae: Crotalinae) como modelo experimental para o estudo do envolvimento de peptidases na sobrevida de espermatozóides
• Beneficiário: Camila Eduardo Marinho
• Modalidade de apoio: Bolsas no Brasil - Mestrado