| Full text | |
| Author(s): |
Marinho, Camila Eduardo
[1, 2]
;
Almeida Santos, Selma Maria
[3]
;
Yamasaki, Simone Cristina
[1]
;
Silveira, Paulo Flavio
[1]
Total Authors: 4
|
| Affiliation: | [1] Inst Butantan, Pharmacol Lab, BR-05503900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Biociencias, Dept Physiol, BR-05508900 Sao Paulo - Brazil
[3] Inst Butantan, Lab Ecol & Evolut, BR-05503900 Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL; v. 179, n. 5, p. 635-642, JUL 2009. |
| Web of Science Citations: | 5 |
| Abstract | |
To understand the role of peptidases in seminal physiology of Crotalus durissus terrificus, intra- and inter-seasonal activity levels of acid (APA), basic (APB), puromycin-sensitive (APN-PS) and puromycin-insensitive neutral (APN-PI), cystyl (CAP), dipeptidyl-IV (DPPIV), type-1 pyroglutamyl (PAP-I) and prolyl-imino (PIP) aminopeptidases as well as prolyl endopeptidase (POP) were evaluated in soluble (SF) and/or membrane-bound (MF) fractions of semen collected from the ductus deferens of the male reproductive tract and from the posterior portion of the uterus. Seminal APB, PIP and POP were detected in SF, while other peptidases were detected in SF and MF. Only the convoluted posterior uterus in winter and autumn had semen. Relative to other examined peptidases, in general, APN-PI, APN-PS and APB activities were predominant in the semen from the uterus and throughout the year in the semen from the ductus deferens, suggesting their great relevance in the seminal physiology of C. d. terrificus. The levels of peptidase activities in the ductus deferens semen varied seasonally and were different from those of semen in the uterus, suggesting that their modulatory actions on susceptible peptides are integrated to the male reproductive cycle events and spermatozoa viability of this snake. (AU) | |
| FAPESP's process: | 05/03745-0 - The rattlesnake Crotalus durissus terrificus (Viperidae: Crotalinae) as an experimental model to study the involvement of peptidases in the survival of spermatozoids |
| Grantee: | Camila Eduardo Marinho |
| Support type: | Scholarships in Brazil - Master |
| FAPESP's process: | 07/06829-6 - Effects of Apis mellifera venom on anti-type II collagen antibody, interleukin 6 and peptidases of synovia, synovium and circulating leukocytes in rats with type-II collagen-induced arthritis |
| Grantee: | Simone Cristina Yamasaki |
| Support type: | Scholarships in Brazil - Master |