Anti-angiogenic effects of pterogynidine alkaloid isolated from Alchornea glandulosa

Autor(es)

Lopes, Flavia C. M. ^[1] ; Rocha, Ana ^[2] ; Pirraco, Ana ^[2] ; Regasini, Luis O. ^[3] ; Silva, Dulce H. S. ^[3] ; Bolzani, Vanderlan S. ^[3] ; Azevedo, Isabel ^[2] ; Carlos, Iracilda Z. ^[1] ; Soares, Raquel ^[2]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, UNESP, Dept Clin Anal, Fac Pharmaceut Sci Araraquara, BR-14801902 Sao Paulo - Brazil

^[2] Univ Porto, Fac Med, Dept Biochem FCT U38, Oporto - Portugal

^[3] Sao Paulo State Univ, UNESP, Dept Organ Chem, Araraquara Inst Chem, BR-14801902 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE; v. 9, MAY 22 2009.
• Citações Web of Science: 15

Resumo

Background: Angiogenesis, a complex multistep process that comprehends proliferation, migration and anastomosis of endothelial cells (EC), has a major role in the development of pathologic conditions such as inflammatory diseases, tumor growth and metastasis. Brazilian flora, the most diverse in the world, is an interesting spot to prospect for new chemical leads, being an important source of new anticancer drugs. Plant-derived alkaloids have traditionally been of interest due to their pronounced physiological activities. We investigated the anti-angiogenic potential of the naturally occurring guanidine alkaloid pterogynidine (Pt) isolated from the Brazilian plant Alchornea glandulosa. The purpose of this study was to examine which features of the angiogenic process could be disturbed by Pt. Methods: Human umbilical vein endothelial cells (HUVEC) were incubated with 8 mu M Pt and cell viability, proliferation, apoptosis, invasion and capillary-like structures formation were addressed. Nuclear factor kappa B (NF kappa B), a transcription factor implicated in these processes, was also evaluated in HUVEC incubated with Pt. Quantifications were expressed as mean +/- SD of five independent experiments and one-way analysis of variance (ANOVA) followed by the Dunnet test was used. Results: A significant decrease in proliferation and invasion capacity and an effective increase in apoptosis as assessed by bromodeoxyuridine (BrdU), double-chamber and terminal transferase dUTP nick end labeling (TUNEL) assay, respectively, have been found. Pt also led to a drastic reduction in the number of capillary-like structures formation when HUVEC were cultured on growth factor reduced-Matrigel (GFR-Matrigel) coated plates. In addition, incubation of HUVEC with Pt resulted in reduced NF kappa B activity. Conclusion: These findings emphasize the potential use of Pt against pathological situations where angiogenesis is stimulated as tumor development. (AU)

• Processo FAPESP: 03/02176-7 - Conservação e uso sustentável da diversidade do Cerrado e da Mata Atlântica: diversidade química e prospecção de medicamentos potenciais - fase II
• Beneficiário: Vanderlan da Silva Bolzani
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático