Phylogenetic classification of human papillomavirus genotypes in high-grade cervical intraepithelial neoplasia in women from a densely populated Brazilian urban region

Autor(es)

Pitta, Denise Rocha ^[1] ; Sarian, Luis Otavio ^[1] ; Campos, Elisabete Aparecida ^[1] ; Rabelo-Santos, Silvia Helena ^[2] ; Syrjanen, Kari ^[3] ; Derchain, Sophie Francoise ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Obstet & Gynecol, Campinas, SP - Brazil

^[2] Univ Fed Goias, Sch Pharm, Goiania, Go - Brazil

^[3] Turku Univ Hosp, Dept Radiotherapy & Oncol, FIN-20520 Turku - Finland

• Tipo de documento: Artigo Científico
• Fonte: São Paulo Medical Journal; v. 127, n. 3, p. 122-127, MAY 7 2009.
• Citações Web of Science: 7

Resumo

CONTEXT AND OBJECTIVE: Differences in human papillomavirus (HPV) types may correlate with the biological potential and invasion risk of high-grade cervical intraepithelial neoplasia (CIN 2 and CIN 3). The objective of this study was to determine the relationship between different combinations of HPV types and CIN severity. DESIGN AND SETTING: Cross-sectional study, at Universidade Estadual de Campinas (Unicamp). METHODS: Cervical samples from 106 women treated due to CIN 2 (18) or CIN 3 (88) were examined for specific HPV genotypes using Roche Linear Array(R) (LA-HPV). The proportions of CIN 2 and CIN 3 in groups of women infected with the HPV phylogenetic groups A7 and A9 were compared. Three groups were formed: women with single infections; multiple infections; and the whole sample. RESULTS: Multiple infections were detected in 68 samples (64.7%). The most frequent high-risk genotypes detected (single/multiple) were HPV 16 (57.1%), HPV 58 (24.7%), HPV 33 (15.2%), HPV 52 (13.3%), HPV 31 (10.4%), HPV 51 (7.6%) and HPV 18 (6.6%). Women without infection with HPV species Alpha 9 were less likely to have CIN 3 than were their Alpha 9 HPV-infected counterparts. HPV 16 and/or HPV 18, with or without associations with other viral types, were more frequently found in women with CIN 3 than in those with CIN 2. CONCLUSIONS: The severity of high-grade CIN may be aggravated by the presence of HPV types included in the Alpha 9 phylogenetic classification and by infections including HPV 16 and 18, singly or in combination with other HPV genotypes. (AU)

• Processo FAPESP: 04/09309-5 - Recidiva da neoplasia intra-epitelial cervical após conização diatérmica em mulheres com infecção pelo papilomavírus humano (HPV), em função da expressão de proteínas marcadoras da regulação do ciclo celular, da apoptose e com participação na invasividade tumoral e neo-angiogênese
• Beneficiário: Sophie Françoise Mauricette Derchain
• Modalidade de apoio: Auxílio à Pesquisa - Regular