Use of the DNA Checkerboard hybridization method for detection and quantitation of Candida species in oral microbiota

Autor(es)

do Nascimento, Cassio ^[1] ; de Albuquerque Junior, Rubens Ferreira ^[1] ; Mardegan Issa, Joao Paulo ^[1] ; Ito, Izabel Yoko ^[2] ; Lovato da Silva, Claudia Helena ^[1] ; Oliveira Paranhos, Helena de Freitas ^[1] ; de Souza, Raphael Freitas ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Dental Sch, Dept Dent Mat & Prosthodont, BR-14040904 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Toxicol & Bromatol Clin Anal, Fac Pharmaceut Sci Ribeirao Preto, BR-14040903 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Canadian Journal of Microbiology; v. 55, n. 5, p. 622-626, MAY 2009.
• Citações Web of Science: 10

Resumo

The DNA Checkerboard method enables the simultaneous identification of distinct microorganisms in a large number of samples and employs up to 45 whole genomic DNA probes to gram-negative and gram-positive bacterial species present in subgingival biofilms. Collectively, they account for 55%-60% of the bacteria in subgingival biofilms. In this study, we present the DNA Checkerboard hybridization as an alternative method for the detection and quantitation of Candida species in oral cavities. Our results reveal that DNA Checkerboard is sensitive enough and constitutes a powerful and appropriate method for detecting and quantifying Candida species found in the oral cavity. (AU)

• Processo FAPESP: 06/56194-4 - Estudo randomizado e controlado do uso caseiro de um evidenciador de biofilme sobre a higiene de proteses totais.
• Beneficiário: Raphael Freitas de Souza
• Modalidade de apoio: Auxílio à Pesquisa - Regular