| Texto completo | |
| Autor(es): |
Travensolo, Regiane de Fatima
[1, 2]
;
Cecchette Gottardi Costa, Maria Vitoria
[1]
;
Carareto-Alves, Lucia Maria
[1]
;
Carrilho, Emanuel
[2]
;
de Macedo Lemos, Eliana Gertrudes
[1]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Estadual Paulista, Dept Tecnol, BR-14884900 Jaboticabal, SP - Brazil
[2] Univ Sao Paulo, Inst Quim Sao Carlos, BR-1356097 Sao Carlos, SP - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Brazilian Archives of Biology and Technology; v. 52, n. 3, p. 555-566, MAY-JUN 2009. |
| Citações Web of Science: | 1 |
| Resumo | |
DNA Microarray was developed to monitor the expression of many genes from Xylella fastidiosa, allowing the side by-side comparison of two situations in a single experiment. The experiments were performed using X. fastidiosa cells grown in two culture media: BCYE and XDM2. The primers were synthesized, spotted onto glass slides and the array was hybridized against fluorescently labeled cDNAs. The emitted signals were quantified, normalized and the data were statistically analyzed to verify the differentially expressed genes. According to the data, 104 genes were differentially expressed in XDM2 and 30 genes in BCYE media. The present study showed that DNA microarray technique efficiently differentiate the expressed genes under different conditions. (AU) | |
| Processo FAPESP: | 00/06289-2 - Análise da expressão de genes de patogenicidade, virulência e adaptação da bactéria Xylella fastidiosa utilizando DNA microarray |
| Beneficiário: | Regiane de Fátima Travensolo |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |