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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Production of DNA microarray and expression analysis of genes from Xylella fastidiosa in different culture media

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Author(s):
Travensolo, Regiane de Fatima [1, 2] ; Cecchette Gottardi Costa, Maria Vitoria [1] ; Carareto-Alves, Lucia Maria [1] ; Carrilho, Emanuel [2] ; de Macedo Lemos, Eliana Gertrudes [1]
Total Authors: 5
Affiliation:
[1] Univ Estadual Paulista, Dept Tecnol, BR-14884900 Jaboticabal, SP - Brazil
[2] Univ Sao Paulo, Inst Quim Sao Carlos, BR-1356097 Sao Carlos, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Brazilian Archives of Biology and Technology; v. 52, n. 3, p. 555-566, MAY-JUN 2009.
Web of Science Citations: 1
Abstract

DNA Microarray was developed to monitor the expression of many genes from Xylella fastidiosa, allowing the side by-side comparison of two situations in a single experiment. The experiments were performed using X. fastidiosa cells grown in two culture media: BCYE and XDM2. The primers were synthesized, spotted onto glass slides and the array was hybridized against fluorescently labeled cDNAs. The emitted signals were quantified, normalized and the data were statistically analyzed to verify the differentially expressed genes. According to the data, 104 genes were differentially expressed in XDM2 and 30 genes in BCYE media. The present study showed that DNA microarray technique efficiently differentiate the expressed genes under different conditions. (AU)