Determination of Matrix Metalloproteinases in Human Radicular Dentin

Autor(es)

Santos, Juliana ^[1] ; Carrilho, Marcela ^{[2, 3]} ; Tervahartiala, Taina ^{[4, 5]} ; Sorsa, Timo ^{[4, 5]} ; Breschi, Lorenzo ^[6] ; Mazzoni, Annalisa ^[7] ; Pashley, David ^[8] ; Tay, Franklin ^[8] ; Ferraz, Caio ^[1] ; Tjaderhane, Leo ^{[9, 10]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Restorat Dent, Endodont Area, Piracicaba Dent Sch, Piracicaba, SP - Brazil

^[2] Univ Estadual Campinas, Dept Restorat Dent, Dent Mat Area, Piracicaba Dent Sch, Piracicaba, SP - Brazil

^[3] Univ Sao Paulo, GEO UNIBAN, Sch Dent, Sao Paulo - Brazil

^[4] Univ Helsinki, Inst Dent, Helsinki - Finland

^[5] Univ Helsinki, Ctr Hosp, Dept Oral & Maxillofacial Dis, Helsinki - Finland

^[6] Univ Trieste, Dept Biomed, Trieste - Italy

^[7] Univ Bologna, Dept SAU&FAL, Bologna - Italy

^[8] Med Coll Georgia, Sch Dent, Dept Oral Biol, Augusta, GA 30912 - USA

^[9] Univ Oulu, Inst Dent, Oulu - Finland

^[10] Oulu Univ Hosp, Oulu - Finland

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ENDODONTICS; v. 35, n. 5, p. 686-689, MAY 2009.
• Citações Web of Science: 65

Resumo

Matrix metalloproteinases (MMPs) are present in sound coronal dentin and may play a role in collagen network degradation in bonded restorations. We investigated whether these enzymes can also be detected in root dentin. Crown and root sections of human teeth were powderized, and dentin proteins were extracted by using guanidine-HCl and EDTA. Extracts were analyzed by zymography and Western blotting for matrix metalloproteinases detection. Zymography revealed gelatinolytic activities in both crown and root dentin samples, corresponding to MMP-2 and MMP-9. MMP-2 was more evident in demineralized root dentin matrix, whereas MMP-9 was mostly extracted from the mineralized compartment of dentin and presented overall lower levels. Western blot analysis detected MMP-8 equally distributed in crown and root dentin. Because MMPs are also present in radicular dentin, their contribution to the degradation of resin-dentin bonds should be addressed in the development of restorative strategies for the root substrate. (J Endod 2009;35:686-689) (AU)

• Processo FAPESP: 07/54618-4 - Avaliação da atividade de colagenases na degradação da dentina humana tratada por procedimentos restauradores adesivos
• Beneficiário: Marcela Rocha de Oliveira Carrilho
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores