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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Determination of Matrix Metalloproteinases in Human Radicular Dentin

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Author(s):
Santos, Juliana [1] ; Carrilho, Marcela [2, 3] ; Tervahartiala, Taina [4, 5] ; Sorsa, Timo [4, 5] ; Breschi, Lorenzo [6] ; Mazzoni, Annalisa [7] ; Pashley, David [8] ; Tay, Franklin [8] ; Ferraz, Caio [1] ; Tjaderhane, Leo [9, 10]
Total Authors: 10
Affiliation:
[1] Univ Estadual Campinas, Dept Restorat Dent, Endodont Area, Piracicaba Dent Sch, Piracicaba, SP - Brazil
[2] Univ Estadual Campinas, Dept Restorat Dent, Dent Mat Area, Piracicaba Dent Sch, Piracicaba, SP - Brazil
[3] Univ Sao Paulo, GEO UNIBAN, Sch Dent, Sao Paulo - Brazil
[4] Univ Helsinki, Inst Dent, Helsinki - Finland
[5] Univ Helsinki, Ctr Hosp, Dept Oral & Maxillofacial Dis, Helsinki - Finland
[6] Univ Trieste, Dept Biomed, Trieste - Italy
[7] Univ Bologna, Dept SAU&FAL, Bologna - Italy
[8] Med Coll Georgia, Sch Dent, Dept Oral Biol, Augusta, GA 30912 - USA
[9] Univ Oulu, Inst Dent, Oulu - Finland
[10] Oulu Univ Hosp, Oulu - Finland
Total Affiliations: 10
Document type: Journal article
Source: JOURNAL OF ENDODONTICS; v. 35, n. 5, p. 686-689, MAY 2009.
Web of Science Citations: 65
Abstract

Matrix metalloproteinases (MMPs) are present in sound coronal dentin and may play a role in collagen network degradation in bonded restorations. We investigated whether these enzymes can also be detected in root dentin. Crown and root sections of human teeth were powderized, and dentin proteins were extracted by using guanidine-HCl and EDTA. Extracts were analyzed by zymography and Western blotting for matrix metalloproteinases detection. Zymography revealed gelatinolytic activities in both crown and root dentin samples, corresponding to MMP-2 and MMP-9. MMP-2 was more evident in demineralized root dentin matrix, whereas MMP-9 was mostly extracted from the mineralized compartment of dentin and presented overall lower levels. Western blot analysis detected MMP-8 equally distributed in crown and root dentin. Because MMPs are also present in radicular dentin, their contribution to the degradation of resin-dentin bonds should be addressed in the development of restorative strategies for the root substrate. (J Endod 2009;35:686-689) (AU)