RNA interference inhibits yellow fever virus replication in vitro and in vivo

Autor(es)

Pacca, Carolina C. ^{[1, 2]} ; Severino, Adriana A. ^{[1, 2]} ; Mondini, Adriano ^[2] ; Rahal, Paula ^[1] ; D'avila, Solange G. P. ^[3] ; Cordeiro, Jose Antonio ^[4] ; Lelles Nogueira, Mara Correa ^[5] ; Bronzoni, Roberta V. M. ^[2] ; Nogueira, Mauricio L. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Dept Biol, IBILCE UNESP, DR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[2] Fac Med Sao Jose do Rio Preto FAMERP, Lab Pesquisas Virol, Dept Doencas Dermatol Infecciosas & Parasitarias, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil

^[3] Fac Med Sao Jose do Rio Preto FAMERP, Dept Patol & Med Legal, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil

^[4] Fac Med Sao Jose do Rio Preto FAMERP, Dept Epidemiol & Saude Colet, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil

^[5] Fac Med Sao Jose do Rio Preto FAMERP, Microbiol Lab, Dept Doencas Dermatol Infecciosas & Parasitarias, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: VIRUS GENES; v. 38, n. 2, p. 224-231, APR 2009.
• Citações Web of Science: 25

Resumo

RNA interference (RNAi) is a process that is induced by double stranded RNA and involves the degradation of specific sequences of mRNA in the cytoplasm of the eukaryotic cells. It has been used as an antiviral tool against many viruses, including flaviviruses. The genus Flavivirus contains the most important arboviruses in the world, i.e., dengue (DENV) and yellow fever (YFV). In our study, we investigated the in vitro and in vivo effect of RNAi against YFV. Using stable cell lines that expressed RNAi against YFV, the cell lines were able to inhibit as much as 97% of the viral replication. Two constructions (one against NS1 and the other against E region of YFV genome) were able to protect the adult Balb/c mice against YFV challenge. The histopathologic analysis demonstrated an important protection of the central nervous system by RNAi after 10 days of viral challenge. Our data suggests that RNAi is a potential viable therapeutic weapon against yellow fever. (AU)

• Processo FAPESP: 04/11098-2 - Vírus da febre amarela: diagnóstico, aspectos moleculares e interferência de RNA
• Beneficiário: Maurício Lacerda Nogueira
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 05/03334-0 - Desenvolvimento de um sistema de interferencia de rna para inibicao da replicacao do virus da febre amarela.
• Beneficiário: Carolina Colombelli Pacca
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 05/03260-7 - Replicacao do virus da febre amarela: estudo das interacoes entre a proteina ns5 e proteinas celulares.
• Beneficiário: Roberta Vieira de Morais Bronzoni
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado