Frequency and prognostic relevance of cancer testis antigen 45 expression in multiple myeloma

Autor(es)

Andrade, Valeria C. C. ; Vettore, Andre L. ^[1] ; Regis Silva, Maria Regina ^[2] ; Felix, Roberta S. ; Almeida, Manuella S. S. ; de Carvalho, Fabricio ; Zago, Marco Antonio ^[3] ; Caballero, Otavia L. ^[4] ; Simpson, Andrew J. ^[4] ; Colleoni, Gisele W. B. ^[5]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, UNIFESP, Diadema - Brazil

^[2] Univ Fed Sao Paulo, UNIFESP, Dept Pathol, BR-04023900 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Sao Paulo - Brazil

^[4] Ludwig Inst Canc Res, New York, NY - USA

^[5] Univ Fed Sao Paulo, UNIFESP, EPM, Discipline Hematol & Hemotherapy, BR-04023900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Experimental Hematology; v. 37, n. 4, p. 446-449, APR 2009.
• Citações Web of Science: 11

Resumo

Objective. This study aims to analyze the expression of cancer testis antigen 45 (CT45) in normal tissues and in plasma cell disorders and to identify possible associations with clinical data and prognosis in multiple myeloma (MM) patients. Materials and Methods. Expression of CT45 was studied in 20 normal tissues (testis, placenta, skeletal muscle, bladder, lung, spleen, heart, brain and fetal brain, thymus, uterus, stomach, mammary gland, pancreas, prostate, small intestine, kidney, adrenal gland, spinal cord, colon, and one pool of 10 normal bone marrow samples) and bone marrow aspirates from 3 monoclonal gammopathies of undetermined significance, 5 solitary plasmacytomas, 61 newly diagnosed MM patients and MM cell line U266 by reverse transcriptase polymerase chain reaction. Results. CT45 was positive in 3 of 20 (15%) normal tissues tested: lung, brain (both fetal and adult), and spinal cord. Among monoclonal gammopathies, CT45 was positive in 2 of 5 (40%) solitary plasmacytomas bone marrow aspirates, 10 of 61 (16%) MM bone marrow aspirates, and in the U266 MM cell line. Conclusions. We did not find associations between bone marrow histology and CT45 expression. However, we demonstrated for the first time that positive expression of CT45 was associated with poor prognostic (international Staging System) and poor outcomes in MM patients, meaning that CT45-positive cases presented seven times more chance of worse evolution than the negative ones. (C) 2009 ISEH - Society for Hematology and Stem Cells. Published by Elsevier Inc. (AU)

• Processo FAPESP: 04/12855-1 - Analise do perfil de expressao genica pela tecnica de microarrays de oligonucleotideos em subgrupos de pacientes com mieloma multiplo definidos a partir de antigenos especificos de cancer/testiculo (ctas)
• Beneficiário: Valeria Cristina da Costa Andrade
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 04/13213-3 - Estudos de expressao genica em mieloma multiplo: identificacao de marcadores tumorais e possiveis alvos terapeuticos.
• Beneficiário: Gisele Wally Braga Colleoni
• Modalidade de apoio: Auxílio à Pesquisa - Regular