Autologous bone marrow transplantation in a dog with lymphoma: a clinical study

Autor(es)

Buffo de Capua, Maria Luisa ^[1] ; Dela Coleta, Flavia Eiras ^[1] ; Miotto, Mariana Rodrigues ^[1] ; Calazans, Sabryna Gouveia ^[1] ; Godoy, Aline Vieira ^[1] ; Massae Nakage, Ana Paula ^[2] ; Sales Lisbao, Carolina Bonduki ; Daleck, Carlos Roberto ^[3] ; Santana, Aureo Evangelista ^[3]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, FCAV, Dept Clin & Cirugia Vet, Programa Posgrad, Jaboticabal, SP - Brazil

^[2] Ctr Univ Barao Maua, Ribeirao Preto, SP - Brazil

^[3] UNESP, FCAV, Dept Clin & Cirugia Vet, Jaboticabal, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Ciência Rural; v. 39, n. 2, p. 580-584, MAR-APR 2009.
• Citações Web of Science: 0

Resumo

The objective of this study is to provide the first report of bone marrow transplantation (BMT) in dogs in Brazil. A Rottweiler with cutaneous lymphoma was submitted to a twelve-week Madison-Wisconsin chemotherapy protocol followed by autologous bone marrow transplantation. For this, 10mL kg(-1) of bone marrow was collected simultaneously from both iliac crests and cryopreserved in a freezer at -80 degrees C. The conditioning step was performed by administering cyclophosphamide by intravenous route at 400mg m(-2). Bone marrow was reinfused after defrosting in a water bath at 37 degrees C. Bone marrow nucleated cell counts before and after freezing, showed a small relative loss of nucleated cells (35.10 and 31.80x10(3) mu L(-1), respectively). Cyclophosphamide induced neutropenia which was reverted by a granulocyte colony-stimulating factor (G-CSF) capable of stimulating hematopoetic reconstitution. On the day 360 after transplant the patient was found to be in complete remission. This study indicates that autologous BMT in a dog with lymphoma submitted to myelosuppressive chemotherapy was potentially safe and effective. (AU)

• Processo FAPESP: 06/03300-1 - Efeito do transplante autologo de medula ossea em caes acometidos por linfoma submetidos a mielossupressao quimioterapia.
• Beneficiário: Áureo Evangelista Santana
• Modalidade de apoio: Auxílio à Pesquisa - Regular