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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Autologous bone marrow transplantation in a dog with lymphoma: a clinical study

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Author(s):
Buffo de Capua, Maria Luisa [1] ; Dela Coleta, Flavia Eiras [1] ; Miotto, Mariana Rodrigues [1] ; Calazans, Sabryna Gouveia [1] ; Godoy, Aline Vieira [1] ; Massae Nakage, Ana Paula [2] ; Sales Lisbao, Carolina Bonduki ; Daleck, Carlos Roberto [3] ; Santana, Aureo Evangelista [3]
Total Authors: 9
Affiliation:
[1] Univ Estadual Paulista, UNESP, FCAV, Dept Clin & Cirugia Vet, Programa Posgrad, Jaboticabal, SP - Brazil
[2] Ctr Univ Barao Maua, Ribeirao Preto, SP - Brazil
[3] UNESP, FCAV, Dept Clin & Cirugia Vet, Jaboticabal, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Ciência Rural; v. 39, n. 2, p. 580-584, MAR-APR 2009.
Web of Science Citations: 0
Abstract

The objective of this study is to provide the first report of bone marrow transplantation (BMT) in dogs in Brazil. A Rottweiler with cutaneous lymphoma was submitted to a twelve-week Madison-Wisconsin chemotherapy protocol followed by autologous bone marrow transplantation. For this, 10mL kg(-1) of bone marrow was collected simultaneously from both iliac crests and cryopreserved in a freezer at -80 degrees C. The conditioning step was performed by administering cyclophosphamide by intravenous route at 400mg m(-2). Bone marrow was reinfused after defrosting in a water bath at 37 degrees C. Bone marrow nucleated cell counts before and after freezing, showed a small relative loss of nucleated cells (35.10 and 31.80x10(3) mu L(-1), respectively). Cyclophosphamide induced neutropenia which was reverted by a granulocyte colony-stimulating factor (G-CSF) capable of stimulating hematopoetic reconstitution. On the day 360 after transplant the patient was found to be in complete remission. This study indicates that autologous BMT in a dog with lymphoma submitted to myelosuppressive chemotherapy was potentially safe and effective. (AU)