Cytochrome-b variation in Apis mellifera samples and its association with COI-COII patterns

Autor(es)

Ferreira, Katia M. ^[1] ; Silva, Otavio Lino e ^[1] ; Arias, Maria C. ^[2] ; Del Lama, Marco A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Genet & Evolucao, BR-13565905 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biociencias, Dept Genet & Biol Evolutiva, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Genetica; v. 135, n. 2, p. 149-155, MAR 2009.
• Citações Web of Science: 7

Resumo

Five Mbo I (Mbo-A, Mbo-M, Mbo-C(1), Mbo-C(2) and Mbo-C(3)) and Hinf I (Hinf-1 to Hinf-5) patterns were observed in Apis mellifera samples after restriction of a 485 bp fragment of the mitochondrial cytochrome-b (cyt-b) gene. Associating the cyt-b Restriction fragment length polymorphism (RFLP) pattern of each sample to its respective previously established COI-COII (Dra I sites) pattern, five restriction patterns (Mbo-C(1), Mbo-C(2), Mbo-C(3), Hinf-1 and Hinf-4) were observed in samples of maternal origin associated to the evolutionary branch C. No deletions or insertions were observed and the nucleotide substitution rate was estimated at 5.4%. Higher nucleotide diversity was observed among the branch C-haplotypes when compared with A and M lineages. Further studies are needed to confirm if the cyt-b + COI-COII haplotypes help to assign certain phylogeographic patterns to the branch C and to clarify phylogenetic relationships among A. mellifera subspecies. (AU)

• Processo FAPESP: 03/06342-9 - A composição e a estrutura genética das populações de Apis mellifera (Linnaeus, 1758) do Brasil e da América do Sul resultantes do processo de africanização
• Beneficiário: Marco Antonio Del Lama
• Modalidade de apoio: Auxílio à Pesquisa - Regular