Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cytochrome-b variation in Apis mellifera samples and its association with COI-COII patterns

Full text
Author(s):
Ferreira, Katia M. [1] ; Silva, Otavio Lino e [1] ; Arias, Maria C. [2] ; Del Lama, Marco A. [1]
Total Authors: 4
Affiliation:
[1] Univ Fed Sao Carlos, Dept Genet & Evolucao, BR-13565905 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Biociencias, Dept Genet & Biol Evolutiva, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Genetica; v. 135, n. 2, p. 149-155, MAR 2009.
Web of Science Citations: 7
Abstract

Five Mbo I (Mbo-A, Mbo-M, Mbo-C(1), Mbo-C(2) and Mbo-C(3)) and Hinf I (Hinf-1 to Hinf-5) patterns were observed in Apis mellifera samples after restriction of a 485 bp fragment of the mitochondrial cytochrome-b (cyt-b) gene. Associating the cyt-b Restriction fragment length polymorphism (RFLP) pattern of each sample to its respective previously established COI-COII (Dra I sites) pattern, five restriction patterns (Mbo-C(1), Mbo-C(2), Mbo-C(3), Hinf-1 and Hinf-4) were observed in samples of maternal origin associated to the evolutionary branch C. No deletions or insertions were observed and the nucleotide substitution rate was estimated at 5.4%. Higher nucleotide diversity was observed among the branch C-haplotypes when compared with A and M lineages. Further studies are needed to confirm if the cyt-b + COI-COII haplotypes help to assign certain phylogeographic patterns to the branch C and to clarify phylogenetic relationships among A. mellifera subspecies. (AU)