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Chemical characterization of two samples of Brazilian propolis and their biological properties

Grant number: 13/21813-0
Support type:Regular Research Grants
Duration: April 01, 2014 - March 31, 2016
Field of knowledge:Biological Sciences - Botany - Applied Botany
Principal Investigator:Antonio Salatino
Grantee:Antonio Salatino
Home Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Assoc. researchers:Beatriz Simonsen Stolf ; Caroline Cristina Fernandes da Silva ; Giuseppina Negri ; Maria Luiza Faria Salatino

Abstract

Propolis is a complex mixture of compounds with resinous aspect, produced by honeybees Apis mellifera. The chemical composition of propolis is highly variable, depending mainly on the local flora. Despite de wide chemical variation and diversity of the already known propolis types, practically all of them have revealed a wide spectrum of biological activity, including antibacterial, antifungal, antiprotozoan and cytotoxic, among many other properties. The Brazilian propolis mostly commercialized both in the domestic and international markets is the green propolis, which is produced with resins from Baccharis dracunculifolia (alecrim-do-campo plant). Most of the research done so far about chemistry and biological properties of Brazilian propolis is focused on studies of hydro-alcoholic extracts of green propolis. However, works that have dealt with less polar extracts from this or other types of propolis have uncovered new compounds. The present project intends: 1) to analyze the chemical composition to determinate their anti-HIV and anti-inflammatory activities and to isolate active constituents of a propolis from Canoinhas (SC) and to chemically characterize and to test the phytotoxic properties of a propolis from Viçosa (MG). From the propolis from Canoinhas (SC) the volatile fraction, obtained by steam distillation, and extracts obtained by solvents with increasing polarity (hexane, dichloromethane, ethyl acetate and methanol) will be analyzed by GC-MS and/or HPLC-MS-MS, to provide its chemical profile. At the same time, an ethyl acetate extract will be submitted to a bio-guided isolation of its constituents, by chromatographic methods, in order to obtain isolated compounds with anti-HIV (inhibition of the reverse transcriptase) and anti-inflammatory (inhibition of the expression of the IL-1² and IL-6 proinflammatory cytokines and cyclooxygenase 2 COX-2 mRNA) activities. The volatile fraction and fractionated extracts will be obtained from the propolis from Viçosa (MG) as described above, and its chemical profile will be evalluated by GC-MS and/or HPLC-MS-MS. After that the phytotoxic activity of these extracts will be tested. With this proposal it is expected to discover new botanical source(s) for propolis from Canoinhas (SC), and to obtain extracts and / or isolated compounds possessing anti-inflammatory, anti-HIV or phytotoxic properties. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ARAUJO, CAMILA DO NASCIMENTO; SIVERO MAYWORM, MARCO AURELIO; YATSUDA, REGIANE; NEGRI, GIUSEPPINA; FARIA SALATINO, MARIA LUIZA; SALATINO, ANTONIO; TIMENETSKY, JORGE; CAMPOS, GUILHERME BARRETO. Chemical composition and antimycoplasma activity of a brown propolis from southern Brazil. JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE, APR 2020. Web of Science Citations: 0.
FERREIRA, JOSELENA M.; FERNANDES-SILVA, CAROLINE C.; SALATINO, ANTONIO; NEGRI, GIUSEPPINA; MESSAGE, DEJAIR. New propolis type from north-east Brazil: chemical composition, antioxidant activity and botanical origin. Journal of the Science of Food and Agriculture, v. 97, n. 11, p. 3552-3558, AUG 30 2017. Web of Science Citations: 8.
FERREIRA, JOSELENA M.; FERNANDES-SILVA, CAROLINE C.; SALATINO, ANTONIO; MESSAGE, DEJAIR; NEGRI, GIUSEPPINA. Antioxidant Activity of a Geopropolis from Northeast Brazil: Chemical Characterization and Likely Botanical Origin. Evidence-based Complementary and Alternative Medicine, 2017. Web of Science Citations: 3.

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