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Phenolic compounds, microbiological quality and antimicrobial capacity of dehydrated bee pollen

Grant number: 13/23179-6
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): March 03, 2014
Effective date (End): March 02, 2015
Field of knowledge:Agronomical Sciences - Food Science and Technology - Food Science
Principal researcher:Ligia Bicudo de Almeida Muradian
Grantee:Adriane Alexandre Machado de Melo
Supervisor abroad: Maria Leticia Miranda Fernandes Estevinho
Home Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Research place: Instituto Politécnico de Bragança (IPB), Portugal  
Associated to the scholarship:11/11746-8 - Physicochemical characterization, microbiological quality, biological properties and botanical origin of dehydrated bee pollen, BP.DR


Bee pollen is produced by Apis mellifera bees, collected by beekeepers at the entrance of the hive, processed and marketed. During its production, collection and processing can occur spoilage microorganisms contamination and even pathogenic, but given the slack of studies of microbiological quality and the variety of products found in the country, little is known about the microbiological quality of the national production. Studies suggests that bee pollen is a source of nutrients and bioactive compounds. Its composition is related to botanical origin, soil and climate conditions of the region where it is produced and the type of processing. The aim of this study is to determine the profile of phenolic compounds, microbiological quality and antimicrobial capacity of 70 samples of dehydrated bee pollen from Apis mellifera, collected in different Brazilian States, and also to correlate the phenolic profile with the antimicrobial capacity of the samples. Microbiological analyses will be performed (aerobic mesophilic, psychrophilic and yeast and molds counts, most probable number of total coliforms and Escherichia coli, spores of sulphite-reducing clostridia and presence or absence of Salmonella sp.), antimicrobial capacity against bacteria and yeasts for dilution assays, and identification and quantification of phenolic compounds by high performance liquid chromatography (HPLC) with coupled mass spectrometer. At the end of this study are expected to determine the sanitary quality of the samples, antimicrobial capacity, if there is variation between samples, and to establish a correlation between the antimicrobial capacity and phenolic compounds. Unpublished data will be generated that will assist in the monitoring of quality control, adding value to this product. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MACHADO DE-MELO, ADRIANE ALEXANDRE; ESTEVINHO, LETICIA M.; MOREIRA, MANUELA M.; DELERUE-MATOS, CRISTINA; DE FREITAS, ALEX DA SILVA; BARTH, ORTRUD MONIKA; DE ALMEIDA-MURADIAN, LIGIA BICUDO. A multivariate approach based on physicochemical parameters and biological potential for the botanical and geographical discrimination of Brazilian bee pollen. FOOD BIOSCIENCE, v. 25, p. 91-110, OCT 2018. Web of Science Citations: 0.
MACHADO DE-MELO, ADRIANE ALEXANDRE; ESTEVINHO, LETICIA M.; MOREIRA, MANUELA M.; DELERUE-MATOS, CRISTINA; DE FREITAS, ALEX DA SILVA; BARTH, ORTRUD MONIKA; DE ALMEIDA-MURADIAN, LIGIA BICUDO. Phenolic profile by HPLC-MS, biological potential, and nutritional value of a promising food: Monofloral bee pollen. JOURNAL OF FOOD BIOCHEMISTRY, v. 42, n. 5 OCT 2018. Web of Science Citations: 5.
MACHADO DE-MELO, ADRIANE ALEXANDRE; MIRANDA FERNANDES ESTEVINHO, MARIA LETICIA; GASPAROTTO SATTLER, JOSE AUGUSTO; SOUZA, BIANCA RODRIGUES; FREITAS, ALEX DA SILVA; BARTH, ORTRUD MONIKA; ALMEIDA-MURADIAN, LIGIA BICUDO. Effect of processing conditions on characteristics of dehydrated bee-pollen and correlation between quality parameters. LWT-FOOD SCIENCE AND TECHNOLOGY, v. 65, p. 808-815, JAN 2016. Web of Science Citations: 17.
DE-MELO, A. A. M.; ESTEVINHO, M. L. M. F.; ALMEIDA-MURADIAN, L. B. A diagnosis of the microbiological quality of dehydrated bee-pollen produced in Brazil. Letters in Applied Microbiology, v. 61, n. 5, p. 477-483, NOV 2015. Web of Science Citations: 10.

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