Effect of Low Intensity Helium-Neon (HeNe) Laser Irradiation on Experimental Paracoccidioidomycotic Wound Healing Dynamics

Autor(es)

Ferreira, Maria Carolina ^[1] ; Gameiro, Jacy ^[1] ; Alo Nagib, Patr-cia Resende ^[1] ; Brito, Vania Nieto ^[2] ; Cruz Vasconcellos, Elza da Costa ^[3] ; Verinaud, Liana ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Microbiol & Immunol, Campinas, SP - Brazil

^[2] Lauro de Souza Lima Inst, Bauru, SP - Brazil

^[3] Univ Estadual Campinas, UNICAMP, Inst Phys Gleb Wataghin, Quantum Elect Dept, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Photochemistry and Photobiology; v. 85, n. 1, p. 227-233, JAN-FEB 2009.
• Citações Web of Science: 13

Resumo

The effect of HeNe laser on the extracellular matrix deposition, chemokine expression and angiogenesis in experimental paracoccidioidomycotic lesions was investigated. At days 7, 8 and 9 postinfection the wound of each animal was treated with a 632.8 nm HeNe laser at a dose of 3 J cm(-2). At day 10 postinfection, the wounds were examined by using histologic and immunohistochemical methods. Results revealed that laser-treated lesions were lesser extensive than untreated ones, and composed mainly by macrophages and lymphocytes. High IL-1 beta expression was shown in the untreated group whereas in laser-treated animals the expression was scarce. On the other hand, the expression of CXCL-10 was found to be reduced in untreated animals and quite intensive and well distributed in the laser-treated ones. Also, untreated lesions presented vascular endothelial growth factor (VEGF) in a small area near the center of the lesion and high immunoreactivity for hypoxia-inducible factor-1 (HIF-1), whereas laser-treated lesions expressed VEGF surrounding blood vessels and little immunoreactivity for HIF-1. Laser-treated lesions presented much more reticular fibers and collagen deposition when compared with the untreated lesion. Our results show that laser was efficient in minimizing the local effects observed in paracoccidioidomycosis and can be an efficient tool in the treatment of this infection, accelerating the healing process. (AU)

• Processo FAPESP: 05/55234-0 - Análise dos efeitos do laser HeNe sobre a matriz extracelular e angiogênese em lesões paracoccidiodomicóticas
• Beneficiário: Liana Maria Cardoso Verinaud
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/55233-3 - Analise dos efeitos do laser hene na matriz extracelular e angiogenese de lesoes paracoccidiodomicoticas.
• Beneficiário: Maria Carolina Ferreira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica