Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of Low Intensity Helium-Neon (HeNe) Laser Irradiation on Experimental Paracoccidioidomycotic Wound Healing Dynamics

Full text
Author(s):
Ferreira, Maria Carolina [1] ; Gameiro, Jacy [1] ; Alo Nagib, Patr-cia Resende [1] ; Brito, Vania Nieto [2] ; Cruz Vasconcellos, Elza da Costa [3] ; Verinaud, Liana [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Microbiol & Immunol, Campinas, SP - Brazil
[2] Lauro de Souza Lima Inst, Bauru, SP - Brazil
[3] Univ Estadual Campinas, UNICAMP, Inst Phys Gleb Wataghin, Quantum Elect Dept, Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Photochemistry and Photobiology; v. 85, n. 1, p. 227-233, JAN-FEB 2009.
Web of Science Citations: 13
Abstract

The effect of HeNe laser on the extracellular matrix deposition, chemokine expression and angiogenesis in experimental paracoccidioidomycotic lesions was investigated. At days 7, 8 and 9 postinfection the wound of each animal was treated with a 632.8 nm HeNe laser at a dose of 3 J cm(-2). At day 10 postinfection, the wounds were examined by using histologic and immunohistochemical methods. Results revealed that laser-treated lesions were lesser extensive than untreated ones, and composed mainly by macrophages and lymphocytes. High IL-1 beta expression was shown in the untreated group whereas in laser-treated animals the expression was scarce. On the other hand, the expression of CXCL-10 was found to be reduced in untreated animals and quite intensive and well distributed in the laser-treated ones. Also, untreated lesions presented vascular endothelial growth factor (VEGF) in a small area near the center of the lesion and high immunoreactivity for hypoxia-inducible factor-1 (HIF-1), whereas laser-treated lesions expressed VEGF surrounding blood vessels and little immunoreactivity for HIF-1. Laser-treated lesions presented much more reticular fibers and collagen deposition when compared with the untreated lesion. Our results show that laser was efficient in minimizing the local effects observed in paracoccidioidomycosis and can be an efficient tool in the treatment of this infection, accelerating the healing process. (AU)