Peptidase activities in Crotalus durissus terrificus semen

Autor(es)

Marinho, Camila Eduardo ^{[1, 2]} ; Almeida-Santos, Selma Maria ^[3] ; Carneiro, Sylvia Mendes ^[4] ; Yamasaki, Simone Cristina ^[1] ; Silveira, Paulo Flavio ^[1]

Afiliação do(s) autor(es)

^[1] Inst Butantan, Pharmacol Lab, BR-05503900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biociencias, Dept Physiol, BR-05508900 Sao Paulo - Brazil

^[3] Inst Butantan, Lab Ecol & Evolut, BR-05503900 Sao Paulo - Brazil

^[4] Inst Butantan, Lab Cellular Biol, BR-05503900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Reproduction; v. 136, n. 6, p. 767-776, DEC 2008.
• Citações Web of Science: 2

Resumo

To understand the role of peptidases in seminal physiology of Crotalus durissus terrificus, activity levels of representative enzymes in semen and their sensitivities to inhibitors, cofactors, and peptide hormones were evaluated. The existence of seminal fractions and the association of peptidases with these fractions were also characterized for the first time in snakes. The prominent inhibitors of aminopeptidases (APs) were amastatin for acid, basic, and neutral; bestatin for basic; and diprotin A for dipeptidyl-IV. Cystyl and prolylimino AN were similarly susceptible to the majority of these inhibitors. The basic and neutral were characterized as metallo-peptidases, acid AP was activated by MnCl(2), and cystyl, prolyl-imino, and type I pyroglutamyl were characterized as sulphydryl-dependent APs. Angiotensin II, vasotocin, bradykinin, fertilization-promoting peptide, and TRH altered the majority of these peptidase activities; these peptides are possible substrates and/or modulators of these peptidases. Peptidase activities were found in all seminal fractions: seminal plasma (SP), prostasome-like (PR) structures, and soluble (S-) and membrane-bound fractions (MFs) of spermatozoa. The levels of activity of each peptidase varied among different seminal fractions. In SP, the higher activities were puromycin-insensitive neutral and basic APs. in PR, the higher activity was puromycin-insensitive neutral AP. In spermatozoa, the higher activity in subcellular SF was puromycin-sensitive neutral, while in MF both puromycin-sensitive and -insensitive neutral AN were equally higher than the other examined peptidases. Data suggested that these peptidases, mainly basic and neutral, have a high relevance in regulating seminal functions of C. d. terrificus. (AU)

• Processo FAPESP: 07/06829-6 - Efeitos do veneno de Apis mellifera sobre anticorpo anti-colágeno tipo II, interleucina 6 e peptidases do líquido e membrana sinoviais e de leucócitos circulantes em ratos com artrite induzida por colágeno tipo II
• Beneficiário: Simone Cristina Yamasaki
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 04/14971-9 - Peptidases do semen, da vagina, do utero e do vas deferens: possivel relacao com a sobrevida de espermatozoides da cascavel crotalus durissus terrificus (viperidae: crotalinae).
• Beneficiário: Paulo Flávio Silveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/03745-0 - A cascavel Crotalus durissus terrificus (Viperidae: Crotalinae) como modelo experimental para o estudo do envolvimento de peptidases na sobrevida de espermatozóides
• Beneficiário: Camila Eduardo Marinho
• Modalidade de apoio: Bolsas no Brasil - Mestrado