Sweet Basil (Ocimum basilicum) Extracts Obtained by Supercritical Fluid Extraction (SFE): Global Yields, Chemical Composition, Antioxidant Activity, and Estimation of the Cost of Manufacturing

Autor(es)

Leal, Patricia F. ^[1] ; Maia, Nilson B. ^[2] ; Carmello, Quirino A. C. ^[3] ; Catharino, Rodrigo R. ^[4] ; Eberlin, Marcos N. ^[4] ; Meireles, M. Angela A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Coll Food Engn, LASEFI DEA FEA, BR-13083970 Campinas, SP - Brazil

^[2] IAC, Inst Agron, Ctr Hort, Sao Paulo - Brazil

^[3] Univ Sao Paulo, ESALQ, Dept Solos & Nutr Plantas, BR-09500900 Sao Paulo - Brazil

^[4] Univ Estadual Campinas, UNICAMP, Thomson Mass Spectrometry Lab IQ, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Food and Bioprocess Technology; v. 1, n. 4, p. 326-338, DEC 2008.
• Citações Web of Science: 46

Resumo

In this work, supercritical technology was used to obtain extracts from Ocimum basilicum (sweet basil) with CO(2) and the cosolvent H(2)O at 1, 10, and 20% (w/w). The raw material was obtained from hydroponic cultivation. The extract's global yield isotherms, chemical compositions, antioxidant activity, and cost of manufacturing were determined. The extraction assays were done for pressures of 10 to 30 MPa at 303 to 323 K. The identification of the compounds present in the extracts was made by GC-MS and ESI-MS. The antioxidant activity of extracts was determined using the coupled reaction of beta-carotene and linolenic acid. At 1% of cosolvent, the largest global yield was obtained at 10 MPa and 303 K (2%, dry basis-d.b.); at 10% of cosolvent the largest global yield was obtained at 10 and 15 MPa (11%, d.b.), and at 20% of cosolvent the largest global yield was detected at 30 MPa and 303 K (24%, d.b.). The main components identified in the extracts were eugenol, germacrene-D, epi-alpha-cadinol, malic acid, tartaric acid, ramnose, caffeic acid, quinic acid, kaempferol, caffeoylquinic acid, and kaempferol 3-O-glucoside. Sweet basil extracts exhibited high antioxidant activity compared to beta-carotene. Three types of SFE extracts from sweet basil were produced, for which the estimated cost of manufacturing (class 5 type) varied from US\$ 47.96 to US\$ 1,049.58 per kilogram of dry extract. (AU)

• Processo FAPESP: 04/09310-3 - Estudo comparativo entre os custos de manufatura e as propriedades funcionais de óleos voláteis obtidos por extração supercrítica e destilação por arraste a vapor
• Beneficiário: Patricia Franco Leal
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 02/12001-7 - Extração de compostos com propriedades funcionais usando tecnologia supercrítica: uso de CO2 + H2O
• Beneficiário: Patricia Franco Leal
• Linha de fomento: Bolsas no Brasil - Mestrado
• Processo FAPESP: 99/01962-1 - Tecnologia supercrítica aplicada ao processamento de óleos essenciais, óleos vegetais, corantes, estévia, amido, celulose, etc.
• Beneficiário: Maria Angela de Almeida Meireles Petenate
• Linha de fomento: Auxílio à Pesquisa - Temático