Deleterious effects of immobilization upon rat skeletal muscle: role of creatine supplementation

Autor(es)

Aoki, Marcelo S. ; Lima, W. P. ; Miyabara, Elen H. ; Gouveia, C. H. A. ; Moriscot, Anselmo Sigari ^[5]

• Tipo de documento: Artigo Científico
• Fonte: Clinical Nutrition; v. 23, n. 5, p. 1176-1183, Oct. 2004.
• Área do conhecimento: Ciências Biológicas - Morfologia
• Assunto(s): Sistema musculoesquelético Creatina

Resumo

The aim of the study was to investigate the impact of creatine feeding (5 g kg&#8722;1 body weight day&#8722;1) upon the deleterious adaptations in skeletal muscle induced by immobilization. Male Wistar rats were submitted to hind limb immobilization together with three dietary manipulations: control, supplemented with creatine for 7 days (along with immobilization) and supplemented with creatine for 14 days (7 days before immobilization and together with immobilization). Muscle weight (wet/dry) was determined in the soleus (SOL) and gastrocnemius (GAS). The analysis of lean mass was performed by DEXA and myosin heavy chain (MHC) distribution by SDS-PAGE. After 14 days of creatine loading, immobilized SOL and GAS total creatine content were increased by 25% and 18%, respectively. Regardless of dietary manipulation, the immobilization protocol induced a decrease in the weight of SOL and GAS (P<0.001). However, creatine feeding for 14 days minimized mass loss in the SOL and GAS (P<0.05). Our findings also indicate that creatine supplementation maximizes the expected slow-to-fast MHC shift driven by immobilization (P<0.05). Previous creatine supplementation attenuates muscle wasting induced by immobilization. This effect is associated with the increment of intramuscular creatine content.(AU)

• Processo FAPESP: 03/00431-0 - Papel de calcineurina sobre o trofismo do musculo esqueletico em ratos submetidos a hipocinesia.
• Beneficiário: Anselmo Sigari Moriscot
• Modalidade de apoio: Auxílio à Pesquisa - Regular