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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Deleterious effects of immobilization upon rat skeletal muscle: role of creatine supplementation

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Author(s):
Aoki, Marcelo S. ; Lima, W. P. ; Miyabara, Elen H. ; Gouveia, C. H. A. ; Moriscot, Anselmo Sigari [5]
Total Authors: 5
Document type: Journal article
Source: Clinical Nutrition; v. 23, n. 5, p. 1176-1183, Oct. 2004.
Field of knowledge: Biological Sciences - Morphology
Abstract

The aim of the study was to investigate the impact of creatine feeding (5 g kg&#8722;1 body weight day&#8722;1) upon the deleterious adaptations in skeletal muscle induced by immobilization. Male Wistar rats were submitted to hind limb immobilization together with three dietary manipulations: control, supplemented with creatine for 7 days (along with immobilization) and supplemented with creatine for 14 days (7 days before immobilization and together with immobilization). Muscle weight (wet/dry) was determined in the soleus (SOL) and gastrocnemius (GAS). The analysis of lean mass was performed by DEXA and myosin heavy chain (MHC) distribution by SDS-PAGE. After 14 days of creatine loading, immobilized SOL and GAS total creatine content were increased by 25% and 18%, respectively. Regardless of dietary manipulation, the immobilization protocol induced a decrease in the weight of SOL and GAS (P<0.001). However, creatine feeding for 14 days minimized mass loss in the SOL and GAS (P<0.05). Our findings also indicate that creatine supplementation maximizes the expected slow-to-fast MHC shift driven by immobilization (P<0.05). Previous creatine supplementation attenuates muscle wasting induced by immobilization. This effect is associated with the increment of intramuscular creatine content.(AU)