Triple helix conformation of botryosphaeran, a (1 -> 3;1 -> 6)-beta-D-glucan produced by Botryosphaeria rhodina MAMB-05

Autor(es)

Giese, Ellen C. ^[1] ; Dekker, Robert F. H. ^[2] ; Barbosa, Aneli M. ^[3] ; da Silva, Roberto ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, IBILCE, Dept Quim & Ciencias Ambientals, BR-15054000 Sao Paulo - Brazil

^[2] Univ Castilla La Mancha, IRICA, E-13071 Ciudad Real - Spain

^[3] Univ Estadual Londrina, CCE, Dept Bioquim & Biotecnol, BR-86051990 Londrina, PR - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Carbohydrate Polymers; v. 74, n. 4, p. 953-956, NOV 21 2008.
• Área do conhecimento: Ciências Exatas e da Terra - Química
• Citações Web of Science: 20
• Assunto(s): Botryosphaeria Hidróxido de sódio Carbono

Resumo

Botryosphaeran, a (1 -> 3;1 -> 6)-beta-D-glucan produced by Botryosphaeria rhodina MAMB-05, was found to be present in a triple helix conformation from helix-coil transition studies using Congo Red. The triple helix conformation was disrupted at increasing alkali concentrations. Conformational changes were also observed using phenanthrene as a fluorescent probe, and the fluorescence intensity decreased 80% in the presence of dimethyl sulfoxide. The results confirmed the triple helix conformation of botryosphaeran, an important property manifesting biological response modifying activity. (C) 2008 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 04/14446-1 - Producao, purificacao e caracterizacao de beta-glucanases de trichoderma herzianum para obtencao de glucooligossacarideos a partir de botriosferana.
• Beneficiário: Ellen Cristine Giese
• Modalidade de apoio: Bolsas no Brasil - Doutorado