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Sodre, Ana Karina M. B.
[1]
;
Rubio, Ileana G. S.
[1]
;
Galrao, Ana Luiza R.
[1]
;
Knobel, Meyer
[1]
;
Tomimori, Eduardo K.
[1]
;
Alves, Venancio A. F.
[2]
;
Kanamura, Cristina T.
[3]
;
Buchpiguel, Carlos A.
[4]
;
Watanabe, Tomoco
[4]
;
Friguglietti, Celso U. M.
[5]
;
Kulcsar, Marco A. V.
[5]
;
Medeiros-Neto, Geraldo
[1]
;
Camargo, Rosalinda Y. A.
[1]
Número total de Autores: 13
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Sch Med, Dept Radiol, Thyroid Study Unit LIM 25, BR-05403900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Med, Dept Radiol, Div Endocrinol, Div Pathol, BR-05403900 Sao Paulo - Brazil
[3] Sao Paulo Publ Hlth Serv, Adolfo Lutz Inst, BR-01246903 Sao Paulo - Brazil
[4] Univ Sao Paulo, Sch Med, Dept Radiol, Nucl Med Ctr, BR-05403900 Sao Paulo - Brazil
[5] Univ Santo Amaro, Dept Head & Neck Surg, BR-04743003 Sao Paulo - Brazil
Número total de Afiliações: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM; v. 93, n. 10, p. 4141-4145, OCT 2008. |
| Citações Web of Science: | 25 |
| Resumo | |
Context: The expression of sodium iodide symporter (NIS) is required for iodide uptake in thyroid cells. Benign and malignant thyroid tumors have low iodide uptake. However, previous studies by RT-PCR or immunohistochemistry have shown divergent results of NIS expression in these nodules. Objective: The objective of the study was to investigate NIS mRNA transcript levels, compare with NIS and TSH receptor proteins expression, and localize the NIS protein in thyroid nodules samples and their surrounding nonnodular tissues (controls). Design: NIS mRNA levels, quantified by real-time RT-PCR, and NIS and TSH receptor proteins, evaluated by immunohistochemistry, were examined in surgical specimens of 12 benign and 13 malignant nodules and control samples. Results: When compared with controls, 83.3% of the benign and 100% of the malignant nodules had significantly lower NIS gene expression. Conversely, 66.7% of the benign and 100% of malignant nodules had stronger intracellular NIS immunostaining than controls. Low gene expression associated with strong intracellular immunostaining was most frequently detected in malignant (100%) than benign nodules (50%; P = 0.005). NIS protein was located at the basolateral membrane in 24% of the control samples, 8.3% of the benign, and 15.4% of the malignant nodules. The percentage of benign nodules with strong TSH receptor positivity (41.6%) was higher than malignant (7.7%). Conclusion: We confirmed that reduced NIS mRNA expression in thyroid malignant nodules is associated with strong intracellular protein staining and may be related to the inability of the NIS protein to migrate to the cellular basolateral membrane. These results may explain the low iodide uptake of malignant nodules. (AU) | |
| Processo FAPESP: | 03/08900-9 - Expressão e localização celular do simportador sódio-iodo (NIS) nos tumores tiroidianos benignos e malignos e nos tecidos tireoidianos normais através de exame imunohistoquímico: correlação com a quantificação de RNA mensageiro por RT-PCR em tempo real |
| Beneficiário: | Geraldo Antônio de Medeiros Neto |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |