Protein-Energy Malnutrition Modifies the Production of Interleukin-10 in Response to Lipopolysaccharide (LPS) in a Murine Model

Autor(es)

Fock, Ricardo Ambrosio ^[1] ; Vinolo, Marco Aurelio Ramirez ^[1] ; Crisma, Amanda Rabello ^[1] ; Nakajima, Karina ^[1] ; Rogero, Marcelo Macedo ^[2] ; Borelli, Primavera ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Expt Hematol Lab, Dept Clin & Toxicol Anal, BR-05508900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Food & Expt Nutr, Sch Pharmaceut Sci, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY; v. 54, n. 5, p. 371-377, OCT 2008.
• Citações Web of Science: 25

Resumo

Malnutrition modifies resistance to infection by impairing a number of physiological processes including hematopoesis and the immune response. In this study, we examined the production of Interleukin-4 (IL-4) and IL-10 in response to lipopolysaccharide (LPS) and also evaluated the cellularity of the blood, bone marrow, and spleen in a mouse model of protein-energy malnutrition. Two-month-old male Swiss mice were subjected to protein-energy malnutrition (PEM) with a low-protein diet (4%) as compared to the control diet (20%). When the experimental group lost approximately 20% of their original body weight, the animals from both groups received 1.25 mu g of LPS intravenously. The Cells ill the blood, bone marrow, and spleen were counted, and circulating levels of IL-4 and IL-10 were evaluated in animals stimulated with LPS. Cells from the spleen, bone marrow, and peritoneal cavity of non-inoculated animals were collected for Culture to evaluate the production of IL-4 and IL-10 after stimulating these cells with 1.25 mu g of LPS in vitro. Malnourished animals presented leucopenia and a severe reduction in bone marrow, spleen, and peritoneal cavity cellularity before and after Stimulus with LPS. The circulating levels of IL-10 were increased in malnourished animals inoculated with LPS when compared to control animals, although the levels of IL-4 did not differ. In cells cultured with LPS, we observed high levels of IL-10 in the bone marrow cells of malnourished animals. These findings suggest that malnourished mice present a deficient immune response to LPS. These alterations may be partly responsible for the immunodeficiency observed in these malnourished mice. (AU)

• Processo FAPESP: 03/07322-1 - Carência proteica: avaliação da síntese de citocinas em um modelo septicêmico: participação do receptor de LPS e do fator NFkappaB
• Beneficiário: Primavera Borelli Garcia
• Modalidade de apoio: Auxílio à Pesquisa - Regular