| Full text | |
| Author(s): |
Fock, Ricardo Ambrosio
[1]
;
Vinolo, Marco Aurelio Ramirez
[1]
;
Crisma, Amanda Rabello
[1]
;
Nakajima, Karina
[1]
;
Rogero, Marcelo Macedo
[2]
;
Borelli, Primavera
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Expt Hematol Lab, Dept Clin & Toxicol Anal, BR-05508900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Food & Expt Nutr, Sch Pharmaceut Sci, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY; v. 54, n. 5, p. 371-377, OCT 2008. |
| Web of Science Citations: | 25 |
| Abstract | |
Malnutrition modifies resistance to infection by impairing a number of physiological processes including hematopoesis and the immune response. In this study, we examined the production of Interleukin-4 (IL-4) and IL-10 in response to lipopolysaccharide (LPS) and also evaluated the cellularity of the blood, bone marrow, and spleen in a mouse model of protein-energy malnutrition. Two-month-old male Swiss mice were subjected to protein-energy malnutrition (PEM) with a low-protein diet (4%) as compared to the control diet (20%). When the experimental group lost approximately 20% of their original body weight, the animals from both groups received 1.25 mu g of LPS intravenously. The Cells ill the blood, bone marrow, and spleen were counted, and circulating levels of IL-4 and IL-10 were evaluated in animals stimulated with LPS. Cells from the spleen, bone marrow, and peritoneal cavity of non-inoculated animals were collected for Culture to evaluate the production of IL-4 and IL-10 after stimulating these cells with 1.25 mu g of LPS in vitro. Malnourished animals presented leucopenia and a severe reduction in bone marrow, spleen, and peritoneal cavity cellularity before and after Stimulus with LPS. The circulating levels of IL-10 were increased in malnourished animals inoculated with LPS when compared to control animals, although the levels of IL-4 did not differ. In cells cultured with LPS, we observed high levels of IL-10 in the bone marrow cells of malnourished animals. These findings suggest that malnourished mice present a deficient immune response to LPS. These alterations may be partly responsible for the immunodeficiency observed in these malnourished mice. (AU) | |