Additive effect of P10 immunization and chemotherapy in anergic mice challenged intratracheally with virulent yeasts of Paracoccidioides brasiliensis

Autor(es)

Marques, Alexandre F. ^[1] ; da Silva, Marcelo B. ^[1] ; Juliano, Maria A. P. ^[2] ; Munhoz, Julian E. ^[1] ; Travassos, Luiz R. ^[3] ; Taborda, Carlos P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Microbiol, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Biophys, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Microbes and Infection; v. 10, n. 12-13, p. 1251-1258, OCT 2008.
• Citações Web of Science: 34

Resumo

Paracoccidioidomycosis is a systemic granulomatous disease manifested in the acute/subacute or chronic forms. The anergic cases of the acute/subacute form are most severe, leading to death threatening conditions. Drug treatment is required to control the disease but the response in anergic patients is generally poor. A 15-mer peptide from the major diagnostic antigen gp43, named P10, induces a T-CD4(+) helper-1 immune response in mice of different haplotypes and protects against intratracheal challenge with virulent P. brasiliensis. Presently, P10 immunization and chemotherapy were associated in an attempt to improve antifungal treatment in Balb/c mice made anergic by adding dexamethasone to the drinking water. The combined drug/peptide treatment significantly reduced the lung CFUs in infected anergic mice, largely preserved lung alveolar structure and prevented fungal dissemination to liver and spleen. Results recommend that a P10-based vaccine should be associated to chemotherapy for improved treatment of paracoccidioidomycosis aiming especially at anergic cases. (C) 2008 Elsevier Masson SAS. All rights reserved. (AU)

• Processo FAPESP: 06/50634-2 - Peptídeos e peptidases: atividades biológicas em doenças infecciosas e câncer
• Beneficiário: Luiz Rodolpho Raja Gabaglia Travassos
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 07/07588-2 - Eficácia e função biológica de anticorpos monoclonais gerados contra antígenos de parede e exoantígenos de Paracoccidioides brasiliensis na infecção experimental
• Beneficiário: Carlos Pelleschi Taborda
• Modalidade de apoio: Auxílio à Pesquisa - Regular