Characterization of Leishmania (Leishmania) amazonensis promastigotes resistant to pentamidine

Autor(es)

Coelho, Adriano C. ^[1] ; Gentil, Luciana G. ^[2] ; da Silveira, Jose Franco ^[2] ; Cotrim, Paulo C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Dept Mol Infecciosas & Parasitarias, Inst Trop Med, BR-05403000 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Experimental Parasitology; v. 120, n. 1, p. 98-102, SEP 2008.
• Citações Web of Science: 15

Resumo

Pentamidine is a second-line agent used in the treatment of leishmaniasis and its mode of action and mechanism of resistance is not well understood. It was previously demonstrated that transfection of promastigotes and amastigotes with the ABC transporter PRP1 gene confers resistance to pentamidine. To further clarify this point, we generated Leishmania amazonensis mutants resistant to pentamidine. Our results indicated that this ABC transporter is not associated with pentamidine resistance in lines generated by drug pressure through amplification or overexpression mechanisms of PRP1 gene. (C) 2008 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 06/04656-4 - Papel do transportador abc prp1 de leishmania spp. na resistencia a pentamidina.
• Beneficiário: Paulo Cesar Cotrim
• Linha de fomento: Auxílio à Pesquisa - Regular