Localization and partial characterization of thermostable glucoamylase produced by newly isolated Thermomyces lanuginosus TO3 in submerged fermentation

Autor(es)

Lopes Goncalves, Aline Zorzetto ^[1] ; Azevedo Carvalho, Ana Flavia ^[1] ; da Silva, Roberto ^[1] ; Gomes, Eleni ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Dept Biol, Inst Biociencias Letras & Ciencias Exatas, IBILCE, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Archives of Biology and Technology; v. 51, n. 4, p. 857-865, JUL-AUG 2008.
• Citações Web of Science: 1
• Assunto(s): Fermentação Aspergillus niger alfa-Glucosidases

Resumo

Thermophilic Thermomyces lanuginosus strain TO3 was isolated from compost pile samples and was used for its ability to produce considerable glucoamylase activity when growing in liquid medium at 45ºC with starch as the sole carbon source. Enzyme productivity was high in submerged fermentation (SmF) with maximum activity of 13 U/mL after 168 h of fermentation. Higher quantities of glucose were released when the substrate for enzyme was soluble starch than maltose or maltooligosaccharides were used. The distribution of glucoamylase between the extracellular and cell-associated fractions varied according to fermentation time. Glucoamylase produced from T. lanuginosus TO3 had optimum activity at 65 ºC and good thermostability in the absence of substrate, with a half-life of 6 h at 60 ºC. The enzyme was stable over a wide pH range (4.0-10.0). (AU)

• Processo FAPESP: 02/08355-8 - Melhoramento da termoestabilidade da glucoamilase de aspergillus awamori pela tecnica de pcr mutagenico, purificacao e caracterizacao da enzima.
• Beneficiário: Roberto da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular