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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Localization and partial characterization of thermostable glucoamylase produced by newly isolated Thermomyces lanuginosus TO3 in submerged fermentation

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Author(s):
Lopes Goncalves, Aline Zorzetto [1] ; Azevedo Carvalho, Ana Flavia [1] ; da Silva, Roberto [1] ; Gomes, Eleni [1]
Total Authors: 4
Affiliation:
[1] Univ Estadual Paulista, Dept Biol, Inst Biociencias Letras & Ciencias Exatas, IBILCE, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Brazilian Archives of Biology and Technology; v. 51, n. 4, p. 857-865, JUL-AUG 2008.
Web of Science Citations: 1
Abstract

Thermophilic Thermomyces lanuginosus strain TO3 was isolated from compost pile samples and was used for its ability to produce considerable glucoamylase activity when growing in liquid medium at 45ºC with starch as the sole carbon source. Enzyme productivity was high in submerged fermentation (SmF) with maximum activity of 13 U/mL after 168 h of fermentation. Higher quantities of glucose were released when the substrate for enzyme was soluble starch than maltose or maltooligosaccharides were used. The distribution of glucoamylase between the extracellular and cell-associated fractions varied according to fermentation time. Glucoamylase produced from T. lanuginosus TO3 had optimum activity at 65 ºC and good thermostability in the absence of substrate, with a half-life of 6 h at 60 ºC. The enzyme was stable over a wide pH range (4.0-10.0). (AU)