| Texto completo | |
| Autor(es): |
Figueiredo, L. T. M.
[1]
;
Moreli, M. L.
[1]
;
Borges, A. A.
[1]
;
Figueiredo, G. G.
[1]
;
Souza, R. L. M.
[1]
;
Aquino, V. H.
[2]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] USP, FMRP, Ctr Pesquisa Virol, BR-14040900 Ribeirao Preto, SP - Brazil
[2] USP, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut, BR-14040900 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Brazilian Journal of Medical and Biological Research; v. 41, n. 7, p. 596-599, JUL 2008. |
| Citações Web of Science: | 28 |
| Resumo | |
Hantavirus cardiopulmonary syndrome (HCPS) has been recognized as an important public heath problem. Five hantaviruses associated with HCPS are currently known in Brazil: Juquitiba, Araraquara, Laguna Negra-like, Castelo dos Sonhos, and Anajatuba viruses. The laboratory diagnosis of HCPS is routinely carried out by the detection of anti-hantavirus IgM and/or IgG antibodies. The present study describes the expression of the N protein of a hantavirus detected in the blood sample of an HCPS patient. The entire S segment of the virus was amplified and found to be 1858 nucleotides long, with an open reading frame of 1287 nucleotides that encodes a protein of 429 amino acids. The nucleotide sequence described here showed a high identity with the N protein gene of Araraquara virus. The entire N protein was expressed using the vector pET200D and the Escherichia coli BL21 strain. The expression of the recombinant protein was confirmed by the detection of a 52-kDa protein by Western blot using a pool of human sera obtained from HCPS patients, and by specific IgG detection in five serum samples of HCPS patients tested by ELISA. These results suggest that the recombinant N protein could be used as an antigen for the serological screening of hantavirus infection. (AU) | |
| Processo FAPESP: | 03/03682-3 - Estudos sobre arboviroses e viroses associadas a roedores |
| Beneficiário: | Luiz Tadeu Moraes Figueiredo |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 02/08459-8 - Rede de Diversidade Genética de Vírus (VIRGEN), selecionado para Laboratório L3: projeto: estudos com Hantavirus e vírus da dengue |
| Beneficiário: | Luiz Tadeu Moraes Figueiredo |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |