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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Expression of a hantavirus N protein and its efficacy as antigen in immune assays

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Figueiredo, L. T. M. [1] ; Moreli, M. L. [1] ; Borges, A. A. [1] ; Figueiredo, G. G. [1] ; Souza, R. L. M. [1] ; Aquino, V. H. [2]
Total Authors: 6
[1] USP, FMRP, Ctr Pesquisa Virol, BR-14040900 Ribeirao Preto, SP - Brazil
[2] USP, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut, BR-14040900 Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Brazilian Journal of Medical and Biological Research; v. 41, n. 7, p. 596-599, JUL 2008.
Web of Science Citations: 28

Hantavirus cardiopulmonary syndrome (HCPS) has been recognized as an important public heath problem. Five hantaviruses associated with HCPS are currently known in Brazil: Juquitiba, Araraquara, Laguna Negra-like, Castelo dos Sonhos, and Anajatuba viruses. The laboratory diagnosis of HCPS is routinely carried out by the detection of anti-hantavirus IgM and/or IgG antibodies. The present study describes the expression of the N protein of a hantavirus detected in the blood sample of an HCPS patient. The entire S segment of the virus was amplified and found to be 1858 nucleotides long, with an open reading frame of 1287 nucleotides that encodes a protein of 429 amino acids. The nucleotide sequence described here showed a high identity with the N protein gene of Araraquara virus. The entire N protein was expressed using the vector pET200D and the Escherichia coli BL21 strain. The expression of the recombinant protein was confirmed by the detection of a 52-kDa protein by Western blot using a pool of human sera obtained from HCPS patients, and by specific IgG detection in five serum samples of HCPS patients tested by ELISA. These results suggest that the recombinant N protein could be used as an antigen for the serological screening of hantavirus infection. (AU)

FAPESP's process: 03/03682-3 - Study on Brazilian arboviruses and rodent related viruses
Grantee:Luiz Tadeu Moraes Figueiredo
Support type: Research Projects - Thematic Grants