| Full text | |
| Author(s): |
Figueiredo, L. T. M.
[1]
;
Moreli, M. L.
[1]
;
Borges, A. A.
[1]
;
Figueiredo, G. G.
[1]
;
Souza, R. L. M.
[1]
;
Aquino, V. H.
[2]
Total Authors: 6
|
| Affiliation: | [1] USP, FMRP, Ctr Pesquisa Virol, BR-14040900 Ribeirao Preto, SP - Brazil
[2] USP, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut, BR-14040900 Ribeirao Preto, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Brazilian Journal of Medical and Biological Research; v. 41, n. 7, p. 596-599, JUL 2008. |
| Web of Science Citations: | 28 |
| Abstract | |
Hantavirus cardiopulmonary syndrome (HCPS) has been recognized as an important public heath problem. Five hantaviruses associated with HCPS are currently known in Brazil: Juquitiba, Araraquara, Laguna Negra-like, Castelo dos Sonhos, and Anajatuba viruses. The laboratory diagnosis of HCPS is routinely carried out by the detection of anti-hantavirus IgM and/or IgG antibodies. The present study describes the expression of the N protein of a hantavirus detected in the blood sample of an HCPS patient. The entire S segment of the virus was amplified and found to be 1858 nucleotides long, with an open reading frame of 1287 nucleotides that encodes a protein of 429 amino acids. The nucleotide sequence described here showed a high identity with the N protein gene of Araraquara virus. The entire N protein was expressed using the vector pET200D and the Escherichia coli BL21 strain. The expression of the recombinant protein was confirmed by the detection of a 52-kDa protein by Western blot using a pool of human sera obtained from HCPS patients, and by specific IgG detection in five serum samples of HCPS patients tested by ELISA. These results suggest that the recombinant N protein could be used as an antigen for the serological screening of hantavirus infection. (AU) | |
| FAPESP's process: | 03/03682-3 - Study on Brazilian arboviruses and rodent related viruses |
| Grantee: | Luiz Tadeu Moraes Figueiredo |
| Support Opportunities: | Research Projects - Thematic Grants |