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Detection and ecology of hantavirus among wild small mammals and their ectoparasites

Grant number: 11/06810-9
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): August 01, 2011
Effective date (End): January 31, 2015
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:Luiz Tadeu Moraes Figueiredo
Grantee:Gilberto Sabino dos Santos Junior
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Hantaviruses, family Bunyaviridae, are predominantly rodent-borne pathogens. Within natural reservoir hosts, hantaviruses do not cause obvious pathogenic effects; transmission to humans, however, can lead to hemorrhagic fever with renal syndrome (HFRS) in Eurasia and hantavirus cardiopulmonary syndrome (HCPS) in the American continent. In South America hantaviruses are associated with rodent hosts of the subfamily Sigmodontinae (family Cricetidae), although in Brazil hantavirus genome was detected in bats (Chiroptera). Our goal in this study will be to detect hantavirus infection in wild volant and non volant small mammals and in its ectoparasites in different habitats. We will also verify the dynamics fluctuation, as well as ecological patterns in its natural reservoirs associated with hantavirus infection. And finally, we will assess experimentally the spillover phenomenon in natural reservoirs in our BSL-3 facility. Non-flying small mammals will be live-trapped in 2 grids of 100 Sherman's traps each, placed at 10-m intervals, for 7 - 10 consecutive nights. Grids will be separated by at least 800 m to represent individual populations. At least 800 m from the grids a trap line will be set up with 100 Sherman's, placed 5 to 10-m intervals, to collect animals organs for viral isolation and carcasses for further morphological identification. Traps will be also placed in the trees between 1 to 3 meters to capture arboreal small mammals. Bats will be captured alive for 7 - 15 consecutive nights in mistnets. Captured animals will be identified by morphological features, marked for recapture, when from the grids, and blood will be collected by retro-orbital sinus. Animals captured in the trap lines and mistnets will be sacrificed to collect animals' organs for viral isolation, characterization and spillover assay in our BSL-3 facility. Ectoparasites from all animals will be collected for viral detection and isolation. IgG antibodies to hantavirus going to be detected by indirect ELISA using as antigen a N recombinant protein of Araraquara hantavirus and a RT-PCR will be performed to confirm hantavirus infection. We also will detect hantavirus load in tissues and blood from infected animals. A pool of ectoparasites of each individual animal will be conducted to detect hantavirus genome. Thus, with our results we expect to understand hantavirus infection in its natural reservoirs, and to corroborate the findings from previously studies that environmental degradation and loss of biodiversity may be a risk factor for hantavirus infection in wild animals and it may be causing a spillover phenomenon that would perhaps contribute for a high infection ratio in humans.

Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE SOUZA, WILLIAM MARCIEL; FUMAGALLI, MARCILIO JORGE; SABINO-SANTOS, JR., GILBERTO; MOTTA MAIA, FELIPE GONCALVES; MODHA, SEJAL; TEIXEIRA NUNES, MARCIO ROBERTO; MURCIA, PABLO RAMIRO; MORAES FIGUEIREDO, LUIZ TADEU. A Novel Hepacivirus in Wild Rodents from South America. Viruses-Basel, v. 11, n. 3 MAR 24 2019. Web of Science Citations: 0.
MOTTA MAIA, FELIPE GONCALVES; DE SOUZA, WILLIAM MARCIEL; SABINO-SANTOS, JR., GILBERTO; FUMAGALLI, MARCILIO JORGE; MODHA, SEJAL; MURCIA, PABLO RAMIRO; MORAES FIGUEIREDO, LUIZ TADEU. A novel polyomavirus in sigmodontine rodents from So Paulo State, Brazil. ARCHIVES OF VIROLOGY, v. 163, n. 10, p. 2913-2915, OCT 2018. Web of Science Citations: 0.
SABINO-SANTOS, JR., GILBERTO; MOTTA MAIA, FELIPE GONCALVES; MARTINS, RONALDO BRAGANCA; GAGLIARDI, TALITA BIANCA; DE SOUZA, WILLIAM MARCIEL; MUYLAERT, RENATA LARA; DE SOUZA LUNA, LUCIANO KLEBER; MELO, DANILO MACHADO; CARDOSO, RICARDO DE SOUZA; BARBOSA, NATALIA DA SILVA; PONTELLI, MARJORIE CORNEJO; MAMANI-ZAPANA, PRISCILA ROSSE; VIEIRA, THALLYTA MARIA; MELO, NORMA MARIA; JONSSON, COLLEEN B.; GOODIN, DOUGLAS; SALAZAR-BRAVO, JORGE; PINTO DASILVA, LUIS LAMBERTI; ARRUDA, EURICO; MORAES FIGUEIREDO, LUIZ TADEU. Natural infection of Neotropical bats with hantavirus in Brazil. SCIENTIFIC REPORTS, v. 8, JUN 13 2018. Web of Science Citations: 1.
DE SOUZA, WILLIAM MARCIEL; ROMEIRO, MARILIA FARIGNOLI; SABINO-SANTOS, JR., GILBERTO; MOTTA MAIA, FELIPE GONCALVES; FUMAGALLI, MARCILIO JORGE; MODHA, SEJAL; TEIXEIRA NUNES, MARCIO ROBERTO; MURCIA, PABLO RAMIRO; MORAES FIGUEIREDO, LUIZ TADEU. Novel orthohepeviruses in wild rodents from Sao Paulo State, Brazil. VIROLOGY, v. 519, p. 12-16, JUN 2018. Web of Science Citations: 7.
DE SOUZA, WILLIAM MARCIEL; DENNIS, TRISTAN; FUMAGALLI, MARCILIO JORGE; ARAUJO, JANSEN; SABINO-SANTOS, JR., GILBERTO; MOTTA MAIA, FELIPE GONCALVES; ACRANI, GUSTAVO OLSZANSKI; TORRES CARRASCO, ADRIANO DE OLIVEIRA; ROMEIRO, MARILIA FARIGNOLI; MODHA, SEJAL; VIEIRA, LUIZ CARLOS; OMETTO, TATIANA; QUEIROZ, LUZIA HELENA; DURIGON, EDISON LUIZ; TEIXEIRA NUNES, MIRCIO ROBERTO; MORAES FIGUEIREDO, LUIZ TADEU; GIFFORD, ROBERT JAMES. Novel Parvoviruses from Wild and Domestic Animals in Brazil Provide New Insights into Parvovirus Distribution and Diversity. Viruses-Basel, v. 10, n. 4 APR 2018. Web of Science Citations: 4.
DE SOUZA, WILLIAM MARCIEL; FUMAGALLI, MARCILIO JORGE; DE ARAUJO, JANSEN; SABINO-SANTOS, JR., GILBERTO; MOTTA MAIA, FELIPE GONSALVES; ROMEIRO, MARILIA FARIGNOLI; MODHA, SEJAL; NARDI, MARCELLO SCHIAVO; QUEIROZ, LUZIA HELENA; DURIGON, EDISON LUIZ; TEIXEIRA NUNES, MARCIO ROBERTO; MURCIA, PABLO RAMIRO; MORAES FIGUEIREDO, LUIZ TADEU. Discovery of novel anelloviruses in small mammals expands the host range and diversity of the Anelloviridae. VIROLOGY, v. 514, p. 9-17, JAN 15 2018. Web of Science Citations: 8.
SABINO-SANTOS, JR., GILBERTO; MOTTA MAIA, FELIPE GONCALVES; VIEIRA, THALLYTA MARIA; MUYLAERT, RENATA DE LARA; LIMA, SABRINA MIRANDA; GONCALVES, CRISTIELI BARROS; BARROSO, PATRICIA DOERL; MELO, MARIA NORMA; JONSSON, COLLEEN B.; GOODIN, DOUGLAS; SALAZAR-BRAVO, JORGE; MORAES FIGUEIREDO, LUIZ TADEU. Evidence of Hantavirus Infection among Bats in Brazil. American Journal of Tropical Medicine and Hygiene, v. 93, n. 2, p. 404-406, AUG 2015. Web of Science Citations: 7.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.