Effects of perinatal protein deprivation and recovery on esophageal myenteric plexus

Autor(es)

Greggio, Flavio M. ^[1] ; Fontes, Ricardo B. V. ^[1] ; Maifrino, Laura B. ^[1] ; Castelucci, Patricia ^[1] ; de Souza, Romeu Rodrigues ^[1] ; Liberti, Edson A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Auton Nervous Syst Lab VQM, Dept Anat, Inst Ciencias Biomed, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: WORLD JOURNAL OF GASTROENTEROLOGY; v. 16, n. 5, p. 563-570, FEB 7 2010.
• Citações Web of Science: 10

Resumo

AIM: To evaluate effects of pre- and postnatal protein deprivation and postnatal recovery on the myenteric plexus of the rat esophagus. METHODS: Three groups of young Wistar rats (aged 42 d) were studied: normal-fed (N42), protein-deprived (D42), and protein-recovered (R42). The myenteric neurons of their esophagi were evaluated by histochemical reactions for nicotinamide adenine dinucleotide (NADH), nitrergic neurons (NADPH)-diaphorase and acetylcholinesterase (AChE), immunohistochemical reaction for vasoactive intestinal polypeptide (VIP), and ultrastructural analysis by transmission electron microscopy. RESULTS: The cytoplasms of large and medium neurons from the N42 and R42 groups were intensely reactive for NADH. Only a few large neurons from the D42 group exhibited this aspect. NADPH detected in the D42 group exhibited low reactivity. The AChE reactivity was diffuse in neurons from the D42 and R42 groups. The density of large and small varicosities detected by immunohistochemical staining of VIP was low in ganglia from the D42 group. In many neurons from the D42 group, the double membrane of the nuclear envelope and the perinuclear cisterna were not detectable. NADH and NADPH histochemistry revealed no group differences in the profile of nerve cell perikarya (ranging from 200 to 400 mu m(2)). CONCLUSION: Protein deprivation causes a delay in neuronal maturation but postnatal recovery can almost completely restore the normal morphology of myenteric neurons. (C) 2010 Baishideng. All rights reserved. (AU)

• Processo FAPESP: 04/00746-3 - Análise da distribuição e do código químico dos receptores purinérgicos P2X2, P2X3 e P2X7 do sistema nervoso entérico de animais submetidos a desnutrição protéica pré e pós-natal e a renutrição pós...
• Beneficiário: Patricia Castelucci
• Modalidade de apoio: Auxílio à Pesquisa - Regular