Anti-inflammatory and antinociceptive effects of Baccharis dracunculifolia DC (Asteraceae) in different experimental models

Autor(es)

dos Santos, Diogo A. ^[1] ; Fukui, Murilo de J. ^[2] ; Nanayakkara, N. P. Dhammika ^[3] ; Khan, Shabana I. ^[3] ; Sousa, Joao Paulo B. ^[4] ; Bastos, Jairo K. ^[4] ; Andrade, Sergio F. ^[1] ; da Silva Filho, AdemarA. ^[2] ; Quintao, Nara L. M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Vale Itajai, CCS, Itajai, SC - Brazil

^[2] Univ Franca, Nucleo Pesquisa Ciencias Exatas & Tecnol, Grp Pesquisas Prod Nat, BR-14404600 Franca, SP - Brazil

^[3] Univ Mississippi, Sch Pharm, Pharmaceut Sci Res Inst, Natl Ctr Nat Prod Res, University, MS 38677 - USA

^[4] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14049 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Ethnopharmacology; v. 127, n. 2, p. 543-550, FEB 3 2010.
• Citações Web of Science: 35

Resumo

Aim of the study: The aerial parts of Baccharis dracunculifolia D.C., popularly known as ``alecrim do campo{''} are used in folk medicine as anti-inflammatory. The aim of the present study was to evaluate the anti-inflammatory and antinociceptive activities of the crude hydroalcoholic extract obtained from leaves of Baccharis dracunculifolia (BdE), which have not been reported. Matetials and methods: BdE was analyzed by HPLC and in vivo evaluated (doses ranging from 50 to 400 mg/kg, p.o.) by using the acetic acid-induced abdominal constrictions, paw oedema induced by carrageenan or histamine, overt nociception models using capsaicin, glutamate or phorbol myristate acetate (PMA), formalin-induced nociception and mechanical hypernociception induced by carrageenan or complete Freund adjuvant (CFA). As positive controls it was used paracetamol in both acetic acid and formalin tests; dipyrone in capsaicin, glutamate and PMA-induced nociception; indomethacin in CFA and carrageenan-induced hypernociception models. In addition, the in vitro effects of BdE on COX-2 activity and on the activation of NF-kappa B were also evaluated. Results: BdE (50-400 mg/kg, p.o.) significantly diminished the abdominal constrictions induced by acetic acid, glutamate and CFA. Furthermore, BdE also inhibited the nociceptive responses in both phases of formalin-induced nociception. BdE, administered orally, also produced a long-lasting anti-hypernociceptive effect in the acute model of inflammatory pain induced by carrageenan. It was also observed the inhibition of COX-2 activity by BdE. Conclusion: In summary, the data reported in this work confirmed the traditional anti-inflammatory indications of Baccharis dracunculifolia leaves and provided biological evidences that Baccharis dracunculifolia, like Brazilian green propolis, possess antinociceptive and anti-inflammatory activities. (C) 2009 Elsevier Ireland Ltd. All rights reserved. (AU)

• Processo FAPESP: 06/60132-4 - Investigação das atividades esquistossomicida, tripanocida e leishmanicida de lignanas e neolignanas obtidas por acoplamento oxidativo in vitro de fenilpropanóides
• Beneficiário: Ademar Alves da Silva Filho
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores