Sequencing of E2 and NS5A regions of HCV genotype 3a in Brazilian patients with chronic hepatitis

Autor(es)

Malta, Fernanda de Mello ^[1] ; Moraes de Medeiros-Filho, Jose Eymard ^[1] ; de Azevedo, Raymundo Soares ^[2] ; Goncalves, Luzia ^[3] ; da Silva, Luiz Caetano ^[1] ; Carrilho, Flair Jose ^[1] ; Rebello Pinho, Joao Renato ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Inst Trop Med, Dept Gastroenterol, Lab Gastroenterol & Hepatol Trop, BR-05403000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med, Dept Patol, BR-05403000 Sao Paulo - Brazil

^[3] Univ Nova Lisboa, Inst Higiene & Med Trop, Unidade Epidemiol & Bioestatist, P-1200 Lisbon - Portugal

• Tipo de documento: Artigo Científico
• Fonte: Memórias do Instituto Oswaldo Cruz; v. 105, n. 1, p. 92-98, FEB 2010.
• Citações Web of Science: 6

Resumo

Hepatitis C virus (HCV) is a major cause of liver disease throughout the world. The NS5A and E2 proteins of HCV genotype 1 were reported to inhibit the double-stranded (ds) RNA-dependent protein kinase (PKR), which is involved in the cellular antiviral response induced by interferon (IFN). The response to IFN therapy is quite different between genotypes, with response rates among patients infected with types 2 and 3 that are two-three-fold higher than in patients infected with type 1. Interestingly, a significant percentage of HCV genotype 3-infected patients do not respond to treatment at all. The aim of this paper was to analyse the sequences of fragments of the E2 and NS5A regions from 33 outpatients infected with genotype 3a, including patients that have responded (SVR) or not responded (NR) to treatment. HCV RNA was extracted and amplified with specific primers for the NS5A and E2 regions and the PCR products were then sequenced. The sequences obtained covered amino acids (aa) 636-708 in E2 and in NS5A [including the IFN sensitivity determining region (ISDR), PKR-binding domain and extended V3 region)]. In the E2 and NS5A regions, we did observe aa changes among patients, but these changes were not statistically significant between the SVR and NR groups. In conclusion, our results suggest that the ISDR domain is not predictive of treatment success in patients infected with HCV genotype 3a. (AU)

• Processo FAPESP: 00/11457-1 - Estudo da diversidade genetica do virus da hepatite c e hantavirus circulantes no estado de sao paulo.
• Beneficiário: João Renato Rebello Pinho
• Modalidade de apoio: Auxílio à Pesquisa - Regular