Coxsackievirus 135 induced apoptosis of HeLa cells: Effects on p53 and SUMO

Autor(es)

Gomes, Rogerio ^[1] ; Guerra-Sa, Renata ^[2] ; Arruda, Eurico ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Cell Biol, BR-14049900 Sao Paulo, SP - Brazil

^[2] Univ Fed Ouro Preto, Ouro Preto, MG - Brazil

^[3] Univ Sao Paulo, Sch Med Ribeirao Preto, Virol Res Ctr, BR-14049900 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: VIROLOGY; v. 396, n. 2, p. 256-263, JAN 20 2010.
• Citações Web of Science: 9

Resumo

Coxsackievirus B5 (CVB5), a human enterovirus of the family Picornaviridae, is a frequent cause of acute and chronic human diseases. The pathogenesis of enteroviral infections is not completely understood, and the fate of the CVB5-infected cell has a pivotal role in this process. We have investigated the CVB5-induced apoptosis of HeLa cells and found that it happens by the intrinsic pathway by a mechanism dependent on the ubiquitin-proteasome system, associated with nuclear aggregation of p53. Striking redistribution of both SUMO and UBC9 was noted at 4 h post-infection, simultaneously with a reduction in the levels of the ubiquitin-ligase HDM2. Taken together, these results suggest that CVB5 infection of HeLa cells elicit the intrinsic pathway of apoptosis by MDM2 degradation and p53 activation, destabilizing protein sumoylation, by a mechanism that is dependent on a functional ubiquitin-proteasome system. (C) 2009 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 04/08868-0 - Consolidação do Centro de Microscopia Funcional do Campus da USP/ Ribeirão Preto
• Beneficiário: Roy Edward Larson
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 95/09692-2 - Meningites por enterovírus: estudos de patogênese, imunidade e prevalência em Ribeirão Preto
• Beneficiário: Eurico de Arruda Neto
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 03/02531-1 - Morte celular por apoptose induzida por coxsackie b5: avaliacao da atividade do complexo do proteassoma.
• Beneficiário: Rogério Gomes dos Santos
• Modalidade de apoio: Bolsas no Brasil - Doutorado