Male and female odors induce Fos expression in chemically defined neuronal population

Autor(es)

Donato, Jr., Jose ^{[1, 2]} ; Cavalcante, Judney Cley ^{[1, 3]} ; Silva, Renata Juliana ^[1] ; Teixeira, Aline Stavin ^[1] ; Bittencourt, Jackson Cioni ^{[1, 4]} ; Elias, Carol Fuzeti ^{[1, 4, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Anat, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil

^[2] Univ Texas SW Med Ctr Dallas, Div Hypothalm Res, Dept Internal Med, Dallas, TX 75390 - USA

^[3] Univ Fed Rio Grande do Norte, Dept Morphol, Ctr Biosci, BR-59072970 Natal, RN - Brazil

^[4] Univ Sao Paulo, Ctr Neurosci & Behav, Inst Psychol, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Physiology & Behavior; v. 99, n. 1, p. 67-77, JAN 2010.
• Citações Web of Science: 37

Resumo

Olfactory information modulates innate and social behaviors in rodents and other species. Studies have shown that the medial nucleus of the amygdala (MEA) and the ventral premammillary, nucleus (PMV) are recruited by conspecific odor stimulation. However, the chemical identity of these neurons is not determined. We exposed sexually inexperienced male rats to female or male odors and assessed Fos immunoreactivity (Fos-ir) in neurons expressing NADPH diaphorase activity (NADPHd, a nitric oxide synthase), neuropeptide Urocortin 3, or glutamic acid decarboxylase rnRNA (GAD-67, a GABA-synthesizing enzyme) in the MEA and PMV. Male and female odors elicited Fos-ir in the MEA and PMV neurons, but the number of Fos-immunoreactive neurons was higher following female odor exposure, in both nuclei. We found no difference in odor induced Fos-ir ill the MEA and PMV comparing fed and fasted animals. Ill the MEA, NADPHd neurons colocalized Fos-ir only in response to female odors. In addition, Urocortin 3 neurons comprise a distinct population and they do not express Fos-ir after conspecific odor stimulation. We found that 80% of neurons activated by male odors coexpressed GAD-67 mRNA. Following female odor, 50% of Fos neurons coexpressed GAD-67 rnRNA. The PMV expresses very little GAD-67, and virtually no colocalization with Fos was observed. We found intense NADPHd activity in PMV neurons, some of which coexpressed Fos-ir after exposure to both odors. The majority of the PMV neurons expressing NADPHd colocalized cocaine-and amphetamine-regulated transcript (CART). Our findings suggest that female and male odors engage distinct neuronal populations in the MEA, thereby inducing contextualized behavioral responses according to olfactory cues. In the PMV, NADPHd/CART neurons respond to male and female odors, suggesting a role in neuroendocrine regulation in response to olfactory cues. (C) 2009 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 05/58997-4 - Papel do nucleo pre-mamilar ventral na regulacao da composicao corporal e da densidade ossea.
• Beneficiário: Jose Donato Junior
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 04/13849-5 - Circuitos peptidérgicos envolvidos na organização do comportamento alimentar
• Beneficiário: Jackson Cioni Bittencourt
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 04/00585-0 - Ocorrência de dimorfismo sexual na morfologia, aferências e expressão gênica do núcleo pré-mamilar ventral: análise no animal adulto e durante o desenvolvimento pós-natal
• Beneficiário: Judney Cley Cavalcante
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto