Microbiopsies of Surface Dental Enamel as a Tool to Measure Body Lead Burden

Autor(es)

Kaneshiro Olympio, Kelly Polido ^[1] ; Naozuka, Juliana ^[2] ; Magalhaes, Ana Carolina ^[3] ; de Pera Garcia, Manuel Valentim ^[4] ; de Oliveira, Pedro Vitoriano ^[2] ; Rabelo Buzalaf, Marilia Afonso ^[3] ; Henriques Bechara, Etelvino Jose ^{[5, 6]} ; Risso Guenther, Wanda Maria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Saude Publ, Dept Saude Ambiental, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Quim, Dept Quim Analit, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Ciencias Biol, Fac Odontol Bauru, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Dept Matemat Aplicada, Inst Matemat & Estat, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-01498 Sao Paulo - Brazil

^[6] Univ Fed Sao Paulo, Dept Ciencias Exatas & Terra, Diadema - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES; v. 73, n. 9, p. 627-636, 2010.
• Citações Web of Science: 2

Resumo

Lead (Pb) poisoning is preventable but continues to be a public health problem in several countries. Measuring Pb in the surface dental enamel (SDE) using microbiopsies is a rapid, safe, and painless procedure. There are different protocols to perform these microbiopsies, but the reliability of dental enamel lead levels (DELL) determination is dependent upon biopsy depth (BD). It is established that DELL decrease from the outermost superficial layer to the inner layer of dental enamel. The aim of this study was to determine DELL obtained by two different microbiopsy techniques on SDE termed protocol I and protocol II. Two consecutive enamel layers were removed from the same subject group (n = 138) for both protocols. Protocol I consisted of a biopsied site with a diameter of 4 mm after the application of 10 l HCl for 35 s. Protocol II involved a biopsied site of 1.6 mm diameter after application of 5 l HCl for 20 s. The results demonstrated that there were no significant differences for BD and DELL between homologous teeth using protocol I. However, there was a significant difference between DELL in the first and second layers using both protocols. Further, the BD in protocol II overestimated DELL values. In conclusion, SDE analyzed by microbiopsy is a reliable biomarker in protocol I, but the chemical method to calculate BD in protocol II appeared to be inadequate for measurement of DELL. Thus, DELL could not be compared among studies that used different methodologies for SDE microbiopsies. (AU)

• Processo FAPESP: 06/56530-4 - Estresse redox e carbonílico associado a alfa-aminocetonas e beta-cetoácidos endógenos: mecanismos e biomarcadores
• Beneficiário: Etelvino José Henriques Bechara
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 01/09641-1 - Radicais livres e espécies excitadas em doenças metabólicas e bioluminescência
• Beneficiário: Etelvino José Henriques Bechara
• Modalidade de apoio: Auxílio à Pesquisa - Temático