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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Characterization of the acute inflammatory response in the hybrid tambacu (Piaractus mesopotamicus male x Colossoma macropomum female) (Osteichthyes)

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Autor(es):
Martins, M. L. [1] ; Myiazaki, D. M. Y. [2] ; Tavares-Dias, M. [3] ; Fenerick, Jr., J. [4] ; Onaka, E. M. [5] ; Bozzo, F. R. [6] ; Fujimoto, R. Y. [7] ; Moraes, F. R. [4, 6]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Fed Santa Catarina, Ctr Ciencias Agr, Dept Aquicultura, Lab AQUOS Sanidade Organismos Aquat, BR-88040900 Florianopolis, SC - Brazil
[2] Poli Nutri Alimentos, BR-06273070 Osasco, SP - Brazil
[3] Univ Fed Amazonas, ICB, Dept Ciencias Fisiol, Manaus, Amazonas - Brazil
[4] Univ Estadual Paulista, Ctr Aquicultura, BR-14870000 Jaboticabal, SP - Brazil
[5] Inst Pesca, Nucleo Avancado Pesquisa Tecnol Agronegocio Pesad, BR-15025970 Sao Jose Do Rio Preto, SP - Brazil
[6] Univ Estadual Paulista, FCAV, Dept Patol Vet, BR-14870000 Jaboticabal, SP - Brazil
[7] Fed Univ Para, Nucleo Estudos Costeiros, BR-8600000 Braganca Paulista, SP - Brazil
Número total de Afiliações: 7
Tipo de documento: Artigo Científico
Fonte: Brazilian Journal of Biology; v. 69, n. 3, p. 957-962, AUG 2009.
Citações Web of Science: 9
Resumo

This work evaluated the acute inflammatory response induced by injections of 0.5 mL saline solution (control), 500 mu g carrageenin and 0.5 mL thioglycollate 3% in the swim bladder of juvenile tambacu hybrid. Fish were distributed in three treatments, three replications and acclimated for a period of 10 days before assay. The cell characterization from the inflammatory exudate was performed in Giemsa and PAS stained smears. Carrageenin, injected in fish, showed an increase on the total number of cells in the inflammatory exudate when compared to saline and thioglycollate injected. Whereas, for carrageenin-injected fish, the percentage of thrombocyte was higher than thioglycollate. On the other hand, granulocyte percentage in thioglycollate-injected fish was higher than the ones injected using carrageenin. Carrageenin provoked the highest migration of macrophage to the inflammatory site. The PAS method confirmed the presence of three types of granulocytes: eosinophilic granular cell (EGC) type 1 with the characteristics of a special granulocytic cell commonly found in the circulating blood; EGC type 2 shorter than the last one and neutrophil. This study contributes to a better understanding of the inflammatory response and infectious processes in native fish. (AU)

Processo FAPESP: 00/12566-9 - Influência de dois tipos de estresse sobre a resposta inflamatória induzida pela carragenina e pelo tioglicolato em (Plaractus mesopotamicus HOLMBERG, 1887 macho x Colossoma macropomum CUVIER, 1818 fêmea)
Beneficiário:Maurício Laterça Martins
Modalidade de apoio: Auxílio à Pesquisa - Regular