In vitro and in vivo determination of antioxidant activity and mode of action of isoquercitrin and Hyptis fasciculata

Autor(es)

Silva, C. G. ^{[1, 2]} ; Raulino, R. J. ^[1] ; Cerqueira, D. M. ^[3] ; Mannarino, S. C. ^[1] ; Pereira, M. D. ^[1] ; Panek, A. D. ^[1] ; Silva, J. F. M. ^[2] ; Menezes, F. S. ^[4] ; Eleutherio, E. C. A. ^[1]

Afiliação do(s) autor(es)

^[1] UFRJ, Dept Bioquim, Inst Quim, BR-21949900 Rio De Janeiro - Brazil

^[2] UFRJ, Dept Quim Organ, Inst Quim, BR-21949900 Rio De Janeiro - Brazil

^[3] UFRJ, ICB, Inst Biofis Carlos Chagas Filho, BR-21941590 Rio De Janeiro - Brazil

^[4] Panoz Inst, Sch Pharm & Pharmaceut Sci, Dublin 2 - Ireland

• Tipo de documento: Artigo Científico
• Fonte: Phytomedicine; v. 16, n. 8, p. 761-767, AUG 2009.
• Citações Web of Science: 35

Resumo

Reactive oxygen species (ROS) are thought to underline the process of ageing and the pathogenicity of various diseases, such as neurodegenerative disorders and cancer. The use of traditional medicine is widespread and plants still present a large source of natural antioxidants that might serve as leads for the development of novel drugs. In this paper, the alcoholic extract from leaves of Hyptis fasciculata, a Brazilian medicinal plant, and isoquercitrin, a flavonoid identified in this species, showed to be active as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavengers. The extract of Hyptis fasciculata and isoquercitrin were also able to increase tolerance of the eukaryotic microorganism Saccharomyces cerevisiae to both hydrogen peroxide and menadione, a source of superoxide. Cellular protection was correlated with a decrease in oxidative stress markers, such as levels of ROS, protein carbonylation and lipid peroxidation, confirming the antioxidant potential of Hyptis fasciculata and isoquercitrin. (C) 2009 Elsevier GmbH. All rights reserved. (AU)

• Processo FAPESP: 04/10067-6 - Estresse, transporte e metabolismo de alfa-glicosídios em Saccharomyces cerevisiae
• Beneficiário: Pedro Soares de Araujo
• Modalidade de apoio: Auxílio à Pesquisa - Temático