Fast separation of selective serotonin reuptake inhibitors antidepressants in plasma sample by nonaqueous capillary electrophoresis

Autor(es)

Formenton Catai, Ana Paula ^[1] ; Carrilho, Emanuel ^[2] ; Lancas, Fernando Mauro ^[2] ; Costa Queiroz, Maria Eugenia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Quim, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Chromatography A; v. 1216, n. 30, p. 5779-5782, JUL 24 2009.
• Citações Web of Science: 20

Resumo

A simple, fast, and sensitive liquid-liquid extraction method followed by nonaqueous capillary electrophoresis (LLE/NACE) was developed and validated for Simultaneous determination of four antidepressants (fluoxetine, sertraline, citalopram and paroxetine) in human plasma. Several experimental separation conditions using aqueous and nonaqueous media separation were tested by varying the electrolyte pH value (for aqueous medium) and the ionic strength concentration considering the similar mobility of the compounds. High-resolution separation was achieved with a mixture of 1.25 mol L(-1) of phosphoric acid in acetonitrile. The quantification limits of the LLE/CE method varied between 15 and 30 ng mL(-1), with a relative standard deviation (RSD) lower than 10.3%. The method was successfully applied in therapeutic drug monitoring and should be employed in the evaluation of plasma levels in urgent toxicological analysis. (C) 2009 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 06/04633-4 - Avaliação da técnica in tube SPME/FIACE-UV na análise de antidepressivos em amostras de plasma
• Beneficiário: Ana Paula Formenton Catai
• Modalidade de apoio: Bolsas no Brasil - Doutorado