Proteomic analysis of kidney in rats chronically exposed to fluoride

Autor(es)

Kobayashi, Claudia A. N. ^[1] ; Leite, Aline L. ^[1] ; Silva, Thelma L. ^[1] ; Santos, Lucilene D. ^[2] ; Nogueira, Fabio C. S. ^[3] ; Oliveiraa, Rodrigo C. ^[1] ; Palma, Mario S. ^[2] ; Domont, Gilberto B. ^[3] ; Buzalaf, Marilia A. R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Dent Sch, Bauru, SP - Brazil

^[2] Univ Estadual Paulista, Rio Claro Biosci Inst, Rio Claro, SP - Brazil

^[3] Univ Fed Rio de Janeiro, Inst Chem, Rio De Janeiro - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Chemico-Biological Interactions; v. 180, n. 2, p. 305-311, JUL 15 2009.
• Citações Web of Science: 27

Resumo

Two-dimensional gel electrophoresis (2-DE) was used to better understand alterations in renal metabolism induced by fluoride (F). Three groups of weanling male Wistar rats were treated with drinking water containing 0 (control), 5, or 50 ppm F for 60 days (n=6/group). Kidneys were collected for proteomic and histological (HE) analysis. After protein isolation, renal proteome profiles were examined using 2-DE and Colloidal Coomassie Blue staining. Protein spots with a 2-fold significant difference as detected by quantitative intensity analysis (image Master Platinum software) and t-test (p < 0.05) were excised and analyzed by MALDI-TOF MS (matrix assisted laser desorption ionization-time-of-flight mass spectrometry). The histological analysis revealed no damage in kidneys induced by F, except for a vascular congestion in the 50 ppm F group. Between control vs 50 ppm F, and control vs 5 ppm F groups, 12 and 6 differentially expressed proteins were detected, respectively. Six proteins, mainly related with metabolism, detoxification and housekeeping, were successfully identified. At the high F group, pyruvate carboxylase, a protein involved in the formation of oxaloacetate was found to be downregulated, while enoyl coenzyme A hydratase, involved in fatty acids oxidation, was found to be upregulated. Thus, proteomic analysis can provide new insights into the alterations in renal metabolism after F exposure, even in low doses. (C) 2009 Elsevier Ireland Ltd. All rights reserved. (AU)

• Processo FAPESP: 05/02744-0 - Análise proteômica em fluidos corporais e tecidos moles em ratos submetidos a tratamento com flúor
• Beneficiário: Marília Afonso Rabelo Buzalaf
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/03273-1 - Análise proteômica em urina e rins de ratos submetidos a tratamento crônico com flúor
• Beneficiário: Claudia Ayumi Nakai Kobayashi
• Modalidade de apoio: Bolsas no Brasil - Mestrado