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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Proteomic analysis of kidney in rats chronically exposed to fluoride

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Author(s):
Kobayashi, Claudia A. N. [1] ; Leite, Aline L. [1] ; Silva, Thelma L. [1] ; Santos, Lucilene D. [2] ; Nogueira, Fabio C. S. [3] ; Oliveiraa, Rodrigo C. [1] ; Palma, Mario S. [2] ; Domont, Gilberto B. [3] ; Buzalaf, Marilia A. R. [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Bauru Dent Sch, Bauru, SP - Brazil
[2] Univ Estadual Paulista, Rio Claro Biosci Inst, Rio Claro, SP - Brazil
[3] Univ Fed Rio de Janeiro, Inst Chem, Rio De Janeiro - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Chemico-Biological Interactions; v. 180, n. 2, p. 305-311, JUL 15 2009.
Web of Science Citations: 27
Abstract

Two-dimensional gel electrophoresis (2-DE) was used to better understand alterations in renal metabolism induced by fluoride (F). Three groups of weanling male Wistar rats were treated with drinking water containing 0 (control), 5, or 50 ppm F for 60 days (n=6/group). Kidneys were collected for proteomic and histological (HE) analysis. After protein isolation, renal proteome profiles were examined using 2-DE and Colloidal Coomassie Blue staining. Protein spots with a 2-fold significant difference as detected by quantitative intensity analysis (image Master Platinum software) and t-test (p < 0.05) were excised and analyzed by MALDI-TOF MS (matrix assisted laser desorption ionization-time-of-flight mass spectrometry). The histological analysis revealed no damage in kidneys induced by F, except for a vascular congestion in the 50 ppm F group. Between control vs 50 ppm F, and control vs 5 ppm F groups, 12 and 6 differentially expressed proteins were detected, respectively. Six proteins, mainly related with metabolism, detoxification and housekeeping, were successfully identified. At the high F group, pyruvate carboxylase, a protein involved in the formation of oxaloacetate was found to be downregulated, while enoyl coenzyme A hydratase, involved in fatty acids oxidation, was found to be upregulated. Thus, proteomic analysis can provide new insights into the alterations in renal metabolism after F exposure, even in low doses. (C) 2009 Elsevier Ireland Ltd. All rights reserved. (AU)

FAPESP's process: 05/02744-0 - Proteomic analysis in body fluids and soft tissues in rats submitted to treatment with fluoride
Grantee:Marília Afonso Rabelo Buzalaf
Support Opportunities: Regular Research Grants