| Texto completo | |
| Autor(es): |
Nishikaku, Angela Satie
[1]
;
Scavone, Renata
[1]
;
Sanchez Molina, Raphael Fagnani
[1]
;
Albe, Bernardo Paulo
[1]
;
Cunha, Claudia Da Silva
[1]
;
Burger, Eva
[1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Dept Imunol, Inst Ciencias Biomed, BR-05508900 Sao Paulo - Brazil
Número total de Afiliações: 1
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Medical Mycology; v. 47, n. 5, p. 495-507, 2008. |
| Citações Web of Science: | 5 |
| Resumo | |
The participation of osteopontin (OPN) in Paracoccidioides brasiliensis infected mice, its association to granulomatogenesis, severity of infection, pattern of lesions, nitric oxide (NO) levels and fungal load were evaluated in this investigation. Immunohistochemistry analysis showed marked OPN staining in extracellular matrix and in macrophages and multinucleated giant cells at the center of lesions, suggesting a possible role of OPN in the distribution of these cells within the granulomas. At 15 days post-infection with a virulent P. brasiliensis isolate, OPN(+) cells were more numerous and intensely immunostained in the loose granulomas of susceptible mice than in those of resistant mice. In addition, high fungal loads and low NO levels were observed in susceptible mice. At 120 days after infection, resistant mice had increased total OPN levels (ELISA) and OPN positivity in compact granulomas, higher NO levels and lower fungal loads than susceptible mice. Residual lesions associated with low OPN levels, high NO and control of fungal dissemination were observed in both mouse strains at 120 days post-infection with the slightly virulent fungal isolate. Therefore, OPN could be associated with higher severity of the disease in an early phase of infection and with a degree of control of the progressive infection. (AU) | |
| Processo FAPESP: | 06/60091-6 - Caracterizacao e modulacao do granuloma paracoccidioidomicotico. |
| Beneficiário: | Eva Burger |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 04/14718-1 - Paracoccidioidomicose: acompanhamento de parâmetros de imunidade adquirida e do estado de ativação de fagócitos em camundongos isogênicos suscetíveis, submetidos à terapia antifúngica |
| Beneficiário: | Renata Scavone de Oliveira |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 01/13873-5 - Estudo do envolvimento dos nucleos da rafe no comportamento alimentar: abordagem em ratos submetidos a restricao alimentare e dupla marcacao com trancadores neuronais. |
| Beneficiário: | Maria Inês Nogueira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 00/10647-1 - Avaliação dos componentes da matriz extracelular, de receptores beta1 integrinas e de citocinas nas lesões granulomatosas de camundongos resistentes e suscetíveis à paracoccidioidomicose |
| Beneficiário: | Angela Satie Nishikaku |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |