Pre- and postnatal exposure to ambient levels of urban particulate matter (PM2.5) affects mice spermatogenesis

Autor(es)

Pires, Adriana ^{[1, 2]} ; de Melo, Elizabeth Neves ^[3] ; Mauad, Thais ^{[1, 2]} ; Nascimento Saldiva, Paulo Hilario ^{[1, 2]} ; de Siqueira Bueno, Heloisa Maria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Dept Patol, Lab Poluicao Atmosfer Expt, BR-01246903 Sao Paulo - Brazil

^[2] Inst Nacl Anal Integrada Risco Ambiental, Sao Paulo - Brazil

^[3] Univ Fed Pernambuco, Dept Anat, Ctr Ciencias Biol, Recife, PE - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INHALATION TOXICOLOGY; v. 23, n. 4, p. 237-245, 2011.
• Citações Web of Science: 17

Resumo

This work characterizes the effects of ambient levels of urban particulate matter (PM2.5) from the city of Sao Paulo on spermatogenesis using mice exposed during the embryo-fetal and/or postnatal phases of development. Parental generations (BALB/c mice) were exposed to air pollution in chambers with or without filtering PM2.5 for 4 months. Animals were mated, and half of the 1-day-old offspring were moved between chambers, which yielded prenatal and postnatal groups. Remaining offspring comprised the non-exposed and pre+postnatal exposed groups. After 90 days, the animals were sacrificed for testis collection and weighing. Optical microscopy was used for the morphometric analyses of the cell counts, spermatogenic cycle, proliferation, and apoptosis. Prenatally exposed animals presented reduced body and testicular weight with an increased gonadosomatic index (GSI). Testicular volume also decreased, as well as the tubular diameter in testes of the same animals. Proliferation, apoptosis, and spermatogenic cycle analyses showed no significant differences among groups. However, the tubules at stage VII of pre- and postnatal animals presented a reduced number of elongated spermatids. Pre+postnatal group presented higher spermatid head retention at stages VIII-XII. These results show that ambient levels of PM2.5 from Sao Paulo city affect spermatogenesis by damaging sperm production. (AU)

• Processo FAPESP: 03/10772-9 - O impacto das exposições intrauterina e nas fases iniciais do desenvolvimento pós-natal aos poluentes atmosféricos no desenvolvimento de alterações adversas na vida adulta: estudo experimental em camundongos com ênfase aos sistemas reprodutor e respiratório
• Beneficiário: Paulo Hilário Nascimento Saldiva
• Modalidade de apoio: Auxílio à Pesquisa - Temático